週末不需更換培養基!
在您享受週末的同時,讓Ajinomoto StemFit® 培養基照顧您的iPS細胞!
無飼養層細胞培養基:
Feeder-free medium for ES/iPS cells
StemFit®Basic02 |
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StemFit®Basic04 |
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比較StemFit®Basic02和StemFit®Basic04
StemFit®Basic02 | StemFit®Basic04 | |
Single-cell culture | ○ | ○ |
Xeno-free | ○ | ○ |
Animal-origin free | X | ○ |
Clinical research | X | ○ |
cGMP | X | 準備中 |
number of bottle | 2 | 1 |
培養基使用量少(6孔盤)
搭配任何細胞外基質都有出色的細胞增長性能
人類201B7 iPSCs 生長在飼養層細胞(MEFs)轉換至無飼養層細胞的飼養條StemFit®Basic02或市售培養基A培養在不同的細胞外基質上 (1000 cells/cm2),培養一週的結果。
以單顆細胞(single-cell clone)傳代具有優異的集落形成效
將人類iPSC接種在Matrigel上以StemFit或市售的A或B培養基培養超過3代。然後,將細胞連續稀釋並以1 cell/well 或10 cells/well 接種在塗覆有Matrigel的96孔盤上。3小時後計數細胞數量,並在第7天計數集落。
卓越和穩定的增殖表現
推薦搭配產品:
Cat. No. | Product | Size | Content |
892022 | iMatrix-511 silk | 0.5 mg/ml | 175 g x 1 vials |
892021 | iMatrix-511 silk | 0.5 mg/ml | 175 g x 6 vials |
人類201B7 iPSC接種在iMatrix-511上用StemFit培養4週,週末不換培養基。 將細胞集落解離為單顆細胞並以圖上所列的密度接種。
參考文獻
Nakagawa, Masato, et al. "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells." Scientific reports 4 (2014): 3594.
Morizane, Ryuji, and Joseph V. Bonventre. "Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells." Nature protocols 12.1 (2017): 195.
Miltenyi Biotec提供多種培養基選擇,支持人類MSCs擴增以及分化為脂肪細胞、成骨細胞和軟骨細胞。 MSCs來源可為骨髓、臍帶、脂肪。不同的StemMACS分化培養基適用於分析MSCs的分化能力或進行品管,以及涉及MSCs分化過程的體外研究,包括基因表達和蛋白質分析。
另有提供GMP Grade產品 ! 請洽當區業務 !
(內含500 mL basal media 和 7 mL Supplement)設計用於培養擴增人類骨髓或其他組織來源的MSCs 。
支持人類MSCs分化成脂肪細胞
支持人類MSCs分化成成骨細胞
支持人類MSCs分化成軟骨細胞
人類MSCs在StemMACS AdipoDiff培養基中培養21天後,以Oil Red O染色確認分化成脂肪細胞 |
人類MSCs在StemMACS OsteoDiff培養基中培養10天後,以NBT substrate染色確認分化成成骨細胞 |
人類MSCs在StemMACS ChondroDiff培養基中培養MSC 21天後,軟骨細胞以aggrecan抗體染軟骨蛋白聚糖(紅色),細胞核(藍色) |
Cat. No. | Product | Size |
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130-104-461 | StemMACS Thiazovivin | 1 mg |
130-106-542 | StemMACS Thiazovivin in Solution | 0.5 mg |
130-103-922 | StemMACS Y27632 | 2 mg |
130-104-169 | StemMACS Y27632 | 5 × 2 mg |
130-106-538 | StemMACS Y27632 in Solution | 2 mg |
Sample preparation 樣本製備 ![]() |
Cell separation 細胞分選 ![]() |
Cell analysis 細胞分析 ![]() |
Cell culture 細胞培養 ![]() |
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Sample preparation 樣本製備 |
Products | Capacity/Content/Components | Order No. |
Dead Cell Removal Kit | for 1×109 total cells | 130-090-101 |
MACSmix Tube Rotator |
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130-090-753 |
Pre-Separation Filters (20 µm) | 50 filters | 130-101-812 |
Pre-Separation Filters (30 µm) | 50 filters | 130-041-407 |
Pre-Separation Filters (70 µm) | 50 filters | 130-095-823 |
Umbilical Cord Dissociation Kit, human | for 25 preparations | 130-105-737 |
Cell separation 細胞分選 |
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Products | Capacity/Content/Components | Order No. |
Human cells | ||
Anti-Fibroblast MicroBeads, human | for 1×109 total cells | 130-050-601 |
Anti-MSCA-1 (W8B2) MicroBead Kit, human | for 1×109 total cells | 130-093-583 |
CD11b MicroBeads, human and mouse | for up to 2×109 total cells | 130-049-601 |
CD11b MicroBeads, human and mouse – lyophilized | for up to 2×109 total cells | 130-097-142 |
CD45 MicroBeads, human | for 1×109 total cells | 130-045-801 |
CD105 MicroBeads, human | for 1×109 total cells | 130-051-201 |
CD117 MicroBead Kit, human | for 2×109 total cells | 130-091-332 |
CD146 MicroBead Kit, human | for 1×109 total cells | 130-093-596 |
CD271 MicroBead Kit, human | for 1×109 total cells | 130-099-023 |
CD271 MicroBead Kit (APC), human | for 1×109 total cells | 130-092-283 |
CD271 MicroBead Kit (PE), human | for 1×109 total cells | 130-092-819 |
Lineage Cell Depletion Kit, human | for 1×109 total cells | 130-092-211 |
Mouse cells | ||
Anti-Sca-1 MicroBead Kit (FITC), mouse | for 1×109 total cells | 130-092-529 |
Anti-Ter-119 MicroBeads, mouse | for 2×109 total cells | 130-049-901 |
CD11b MicroBeads, human and mouse | for up to 2×109 total cells | 130-049-601 |
CD11b MicroBeads, human and mouse – lyophilized | for up to 2×109 total cells | 130-097-142 |
CD45 MicroBeads, mouse | for 2×109 total cells | 130-052-301 |
CD45 MicroBeads, mouse – lyophilized | for 2×109 total cells | 130-097-153 |
CD105 MultiSort Kit (PE), mouse | for 1×109 total cells | 130-092-924 |
CD117 MicroBeads, mouse | for 1×109 total cells | 130-091-224 |
CD117 MicroBeads, mouse – lyophilized | for 1×109 total cells | 130-097-146 |
Direct Lineage Cell Depletion Kit, mouse | for 1×109 total cells | 130-110-470 |
Lineage Cell Depletion Kit, mouse | for 1×109 total cells | 130-090-858 |
Cell analysis 細胞分析 |
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Products | Clone | Fluorescent |
Human | ||
Anti-Fibroblast, human | REA165 | FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, Biotin |
Anti-HLA-DR, human | AC122 | FITC, PE, APC, VioBlue, PerCP, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin |
CD45, human | 5B1 | FITC, VioGreen, APC-Vio770, PerCP-Vio700, Biotin |
CD73, human | AD2 | PE, APC, PE-Vio770, Biotin |
CD90, human | DG3 | FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, Biotin |
CD105, human | 43A4E1 | FITC, PE, APC, VioBlue, PE-Vio770, Biotin |
CD117, human | A3C6E2 | PE, APC, PE-Vio770, APC-Vio770, Biotin |
CD146, human | 541-10B2 | FITC, PE, APC, VioBlue, PE-Vio770, Biotin |
CD271 (LNGFR), human | ME20.4-1.H4 | FITC, VioBright FITC, PE, APC, VioBlue, PE-Vio770, Biotin |
Mouse | ||
Anti-Sca-1, mouse | D7 | FITC, PE, APC, Biotin |
Anti-Ter-119, mouse | Ter-119 | FITC, PE, APC, VioBlue, PerCP-Vio700, Biotin |
CD45, mouse | 30F11 | PE, APC, VioBlue, VioGreen, PerCP, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin |
CD90.2, mouse | 30-H12 | FITC, PE, APC, VioBlue, VioGreen, PerCP, PerCP-Vio700, Biotin |
Products | Capacity/Content/Components | Order No. |
MSC Enumeration Kit, human | for 25 tests | 130-106-646 |
MSC Phenotyping Kit, human | for 50 tests | 130-095-198 |
Cell culture 細胞培養 |
Products | Capacity/Content/Components | Order No. |
StemMACS AdipoDiff Media, human | 100 mL | 130-091-677 |
StemMACS ChondroDiff Media, human | 100 mL | 130-091-679 |
StemMACS OsteoDiff Media, human | 100 mL | 130-091-678 |
StemMACS MSC Expansion Media Kit XF, human | 500 mL | 130-104-18 |
StemMACS MSC Expansion Media, human | 500 mL | 130-091-680 |
StemMACS Cryo-Brew | 50 mL | 130-109-558 |
CytoMix – MSC, human | 100 µg | 130-093-552 |
Human BMP-2, research grade | 10 µg | 130-110-923 |
Human BMP-2, research grade | 25 µg | 130-110-922 |
Human BMP-2, premium grade | 10 µg | 130-110-924 |
Human BMP-2, premium grade | 25 µg | 130-110-925 |
Human BMP-2, premium grade | 100 µg | 130-110-926 |
Human BMP-2, premium grade | 1000 µg | 130-110-927 |
Human BMP-7, research grade | 10 µg | 130-093-818 |
Human BMP-7, research grade | 100 µg | 130-103-436 |
Human BMP-7, research grade | 1000 µg | 130-108-988 |
Human EG-VEGF, research grade | 20 µg | 130-093-829 |
Human EGF, research grade | 100 µg | 130-093-825 |
Human EGF, premium grade | 100 µg | 130-097-749 |
Human EGF, premium grade | 500 µg | 130-097-750 |
Human EGF, premium grade | 1000 µg | 130-097-751 |
Human FGF-1, research grade | 10 µg | 130-093-835 |
Human FGF-1, research grade | 25 µg | 130-095-789 |
Human FGF-1, premium grade | 10 µg | 130-095-790 |
Human FGF-1, premium grade | 25 µg | 130-095-763 |
Human FGF-1, premium grade | 100 µg | 130-095-761 |
Human FGF-1, premium grade | 1000 µg | 130-095-756 |
Human FGF-2, research grade | 10 µg | 130-093-837 |
Human FGF-2, research grade | 50 µg | 130-093-838 |
Human FGF-2, premium grade | 10 µg | 130-093-839 |
Human FGF-2, premium grade | 50 µg | 130-093-840 |
Human FGF-2, premium grade | 100 µg | 130-093-564 |
Human FGF-2, premium grade | 200 µg | 130-093-841 |
Human FGF-2, premium grade | 1000 µg | 130-093-842 |
Human FGF-2, premium grade | 2000 µg | 130-093-843 |
Human HGF, research grade | 5 µg | 130-093-871 |
Human HGF, research grade | 25 µg | 130-093-872 |
Human HGF, research grade | 100 µg | 130-103-437 |
Human IGF-1, research grade | 50 µg | 130-093-885 |
Human IGF-1, research grade | 100 µg | 130-093-886 |
Human IGF-1, research grade | 1000 µg | 130-093-887 |
Human IGF-2, research grade | 10 µg | 130-093-888 |
Human IGF-2, research grade | 50 µg | 130-093-889 |
Human IGF-2, research grade | 1000 µg | 130-093-890 |
Human TGF-β1, premium grade | 5 µg | 130-095-067 |
Human TGF-β1, premium grade | 25 µg | 130-095-066 |
Human TGF-β1, premium grade | 100 µg | 130-108-969 |
Human TGF-β1, premium grade | 1000 µg | 130-108-971 |
Human TGF-β2, research grade | 5 µg | 130-094-005 |
Human TGF-β3, research grade | 5 µg (liquid) | 130-094-007 |
Human TGF-β3, research grade | 20 µg (liquid) | 130-094-008 |
Human TGF-β3, research grade | 100 µg (liquid) | 130-108-981 |
Human TNF-α, premium grade | 10 µg | 130-094-014 |
Human TNF-α, research grade | 10 µg | 130-094-015 |
Human TNF-α, research grade | 50 µg | 130-094-017 |
Human TNF-α, research grade | 100 µg | 130-094-018 |
Human TNF-α, research grade | 750 µg | 130-094-019 |
Human TNF-α, research grade | 1000 µg | 130-094-020 |
Human TNF-α, premium grade | 10 µg | 130-094-022 |
Human TNF-α, premium grade | 50 µg | 130-094-023 |
Human TNF-α, premium grade | 100 µg | 130-094-024 |
Human TNF-α, premium grade | 1000 µg | 130-094-562 |
Human VEGF (121 aa), research grade | 10 µg | 130-094-029 |
Human VEGF (121 aa), research grade | 100 µg | 130-094-030 |
Mouse EGF, research grade | 100 µg | 130-094-036 |
Mouse EGF, research grade | 500 µg | 130-094-037 |
Mouse FGF-8b, research grade | 10 µg | 130-096-100 |
Mouse FGF-8b, research grade | 25 µg | 130-096-101 |
Mouse FGF-8b, premium grade | 10 µg | 130-096-102 |
Mouse FGF-8b, premium grade | 25 µg | 130-096-103 |
Mouse FGF-8b, premium grade | 100 µg | 130-096-104 |
Mouse FGF-8b, premium grade | 1000 µg | 130-096-105 |
Mouse TNF-α, research grade | 10 µg | 130-101-688 |
Mouse TNF-α, research grade | 25 µg | 130-101-687 |
Mouse TNF-α, premium grade | 10 µg | 130-101-689 |
Mouse TNF-α, premium grade | 25 µg | 130-101-690 |
Mouse TNF-α, premium grade | 100 µg | 130-101-691 |
Mouse TNF-α, premium grade | 1000 µg | 130-101-692 |
Mouse VEGF (164 aa), research grade | 5 µg | 130-094-086 |
Mouse VEGF (164 aa), research grade | 20 µg | 130-094-087 |
MSC Suppression Inspector, human | 2.5 mL | 130-096-207 |
Rat VEGF-C (C152S), research grade | 5 µg | 130-094-093 |
Rat VEGF-C, research grade | 10 µg | 130-094-092 |