Clone Collections

cDNA Clones

現成/客製化Clone供應商--GeneCopoeia,提供三種不同等級的cDNA Clone,您可依照時間、預算及實驗需求等考量選擇最適合的等級:
 
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Selection Guides
Brochure
 
 


——  種不同等級cDNA Clone的差異  ——

Product
Feature Expression-Ready ORF cDNA Clone ORF cDNA Shuttle Clone Whole Transcript cDNA Template Clone
Protein expression-ready Yes No, need several subcloning steps No, need many subcloning steps (labor intensive)
cDNA insert ORF only, no 5’ and 3’ UTRs ORF only, no 5’ and 3’ UTRs Full-length cDNA with 5’ and 3’ UTRs
Promoter Numerous promoter choices No promoter No promoter
Tag 50+ tag choices No tag No tag
Selection marker Various choices No selection marker No selection marker
Ribosomal binding site Yes (optimized) Yes (optimized) Yes (original)
Coding region fully sequenced Yes Yes Yes
Vector type 100+ vector choices for mammalian, bacterial, yeast, insect, lentiviral, cell free systems ORFEXPRESS - Gateway® PLUS vector (with additional MCS) for recombination or traditional cloning Non-expression, nonshuttle vector Traditional cloning only, no recombination cloning
Need for destination vector Not required, expression ready Need destination expression vector Need empty expression vector


 
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——  用三種不同等級cDNA Clone表現蛋白所需的實驗步驟  ——

 



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OmicsLink™ Expression-Ready ORF cDNA Clones
 
Genecopoeia可提供超過45000個human 和 mouse的 full-length protein-coding ORF cDNA clones。非常適合用在不同細胞系統中進行蛋白表現、純化和功能分析。OmicsLink expression-ready ORF cDNA clones生產自於經序列驗證的full-length cDNA clones或高品質的cDNA libraries,經由GeneCopoeia的RecJoinTM cloning technology建構於表現載體上。您可以要求將感興趣的基因clone進所選的載體中,或著直接使用GeneCopoeia pre-made好的human 和 mouse expression-ready ORF cDNA clones。
 


 
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EZShuttle™  ORF Clones
——  EZShuttle™ Gateway® PLUS ORF Clones  ———————————————————————————
EZShuttle™ Gateway® PLUS ORF Clones的ORF序列兩側具attL1 / attL2位點,可使用EZShuttle™ recombination cloning system 或Gateway® systems進行Insert的轉移。兩側的multiple cloning sites (MCS),亦可允許使用限制酶切割連接方將Insert在不同表現載體上進行轉移。
 
  • 彈性的subcloning選擇 –可使用Gateway、EZshuttle系統或傳統的限制酶切割連接方式轉移Insert。
  • 涵蓋範圍廣 – 可提供25,000個human和20,000個mouse基因
  • 載體具帶有stop codon和不帶stop codon兩種選擇
 
*EZShuttle™ Gateway® PLUS shuttle clones上不帶promotor序列,若您有時間上的考量,想跳過subcloning步驟,我們建議您選擇可直接進行表現的OmicsLink™ Expression-Ready ORF cDNA Clones
 


 
 
——  ORFeome Collaboration Clones  ———————————————————————————————
 
身為ORFeome Collaboration的正式成員,GeneCopoeia提供在Dana Farber Cancer Institute-Center for Cancer Systems Biology、NCBI、WISI/HAVANA group 和 IMAGE Consortium等機構幫助下所建構而成的 ORFeome ORF cDNA clones。
 
  • 建構在 Gateway® entry vectors (僅具att位點,不具MCS)上,ORFs可利用重組方式轉移至任何型態的Gateway® compatible destination expression vector上
  • 不具 multiple cloning sites,無法使用傳統的cloning方式
 
* ORFeome Collaboration ORF cDNA Clones上不帶promotor序列,若您有時間上的考量,想跳過subcloning步驟,我們建議您選擇可直接進行表現的OmicsLink™ Expression-Ready ORF cDNA Clones
 
 


 
 
Full-length whole transcript cDNA template clone collection
GeneCopoeia提供16,000個含帶有5’ 和3’ UTRs 的human full-length whole transcript cDNA template clones。

*此clone需經過繁複的Subcloning步驟才能獲得可表現的construct