Lymphocyte Separation Medium |
Cell separation reagents 細胞分選試劑 │ Manual cell separation 手動細胞分選設備 │
Automated cell separation 全自動細胞分選儀器 │ Cell separation buffers 細胞分選專用緩衝液 │ Selection Strategy 分選策略
Miltenyi Biotec具有成熟的細胞分選技術,包含25年的經驗以及2萬多筆的已發表文獻,為細胞分選技術的黃金標準。MACS technology的細胞分選技術核心,是利用專一性的抗體抗原結合,由抗體上帶有的microBeads受磁場影響而結合到Column上面,接著將Column從Separator取下,進一步利用Rinse Buffer將目標細胞沖提出。
Miltenyi Biotec提供多樣化的Cell separation reagents,自由的選擇最適合的試劑並且配合專用的手動細胞分選設備(Columns與Separators),讓您簡單且快速的完成實驗。面對數量龐大的樣本數,Miltenyi Biotec另有全自動細胞分選儀器(autoMACS Pro Separator與MultiMACS cell24 Separator),減少實驗手動誤差並縮短您的實驗時間。另有Whole blood microbeads以及MACSxpress可以直接使用在人類全血樣本細胞分選的技術,快速且溫和的從血液中拿到您所需要的細胞。Miltenyi Biotec的細胞分選技術可兼容下游實驗,例如: 流式細胞儀分析。
Cell separation reagents
細胞分選試劑
 |
‧ T cell ‧ B cell ‧ NK cell ‧ Dendritic cell ‧ Monocytes and Macrophage
‧ Granulocytes and Myeloid cells ‧ Cytokine-producing cells
‧ Endothlial cells ‧ Tumor cells ‧ Cancer stem cells
‧ Hematopoietic stem cells ‧ Mesenchymal stem cells ‧ ES and iPS cells
‧ Neural cells ‧ Cardiac cells ‧ Any cell types ‧ Various cell types |
Manual cell separation
手動細胞分選設備
 |
MACS Technology:
- Whole microbeads
- Buffy Coat MicroBeads
- REAlease Microbeads
- MACSprep HLA isolation kit
- MACSxpress
|
Automated cell separation
全自動細胞分選儀器
 |
- autoMACS Pro Separator
- MultiMACS cell24 Separator
- Robotic integration
|
Cell separation buffers
細胞分選專用緩衝液
 |
- autoMACS Pro Washing Solution
- autoMACS Rinsing Solution
- autoMACS Running solution – MACS Separation Buffer
- MACS BSA Stock Solution
- AstroMACS Separation Buffer
|
Selection Strategy
分選策略
 |
- Positive Selection
- Untouched Selection
|
根據您的起始材料選擇最適合的試劑套組:
起始
材料 |
Tissue single cell
suspension or PBMC |
Whole blood or
Bone marrow |
Buffy coat |
Whole blood or Spleen |
Whole blood |
試劑
套組 |
Cell separation reagents (microbeads) |
Whole blood microbeads |
Buffy coat microbeads |
MACSprep HLA isolation kits |
MACSxpress |
手動細胞分選 |
MACS columns |
Whole blood column |
Whole blood column |
MACSxpress |
MACSxpress |
自動細胞分選 |
autoMACS pro or
MultiMACS Cell24 |
autoMACS pro or
MultiMACS Cell24 |
MultiMACS Cell24 |
X |
X |
Cell separation reagents 細胞分選試劑
Miltenyi Biotec有最全面的產品組合,試劑可運用在分選不同物種的任何細胞型態,實驗結果具有出色的表現,並且被廣泛的應用於不同的研究領域。可搭配手動細胞分選設備( Columns與Separators )或全自動細胞分選儀器( autoMACS Pro Separator與MultiMACS cell24 Separator ) 。
 |
- Microbeads為超順磁性的奈米粒子(~50 nm)
- 生物可分解且沒有毒性
- 適用於不同物種及任何細胞種類
- 可應用在基礎與臨床研究
|
|
Manual cell separation 手動細胞分選設備
簡單且標準化的實驗流程,輕鬆的完成多種細胞分選,並且具有出色的實驗結果。Miltenyi Biotec提供專一性的Cell separation reagents,並配合上專用的Columns與Separators,快速的得到大量、純度高且存活率高的目標細胞,讓您在實驗桌上完成初步的細胞分選。另有Whole Blood microBeads與MACSxpress可用在人類全血樣本的細胞分選技術。
Columns
 |
- 管柱通透性高,不會有阻塞並傷害細胞
- 提供溫和且有效的細胞分選環境
- 使用最小量的磁性標記既能達到最佳的細胞分選效果,保留大部分的epitope
|
Products: |
Capacity/Content/Components |
Order No. |
CS columns |
- 25 x CS Column
- 25 x 3-way stopcock
- 25 x syringe (10 mL)
- 25 x flow resistor (20G)
- 25 x flow resistor (21G)
- 25 x flow resistor (22G)
- 25 x flow resistor (23G)
|
130-041-305 |
D columns |
- 5 x D Column
- 5 x flow resistor (20G)
- 5 x flow resistor (21G)
- 5 x flow resistor (22G)
- 5 x 3-way stopcock
|
130-041-201 |
Large Cell Columns |
- 25 x Large Cell Column
- 25 x flow resistor (23G)
|
130-042-202 |
LD Columns |
|
130-042-901 |
LS Columns |
|
130-042-401 |
LS Columns plus tubes |
- LS Column (130-042-401)
- 13 mL Tubes (3 x 25)
|
130-041-306 |
MS Columns |
|
130-042-201 |
MS Columns plus tubes |
- MS Columns (130-042-201)
- 5 mL Tubes (3 x 25)
|
130-041-301 |
Whole Blood Column Kit |
- 10 x Whole Blood Columns
- Whole Blood Column Elution Buffer
|
130-093-545 |
XS Columns |
- 5 x XS Column
- 5 x 3-way stopcock
- 5 x syringe (20 mL)
- 5 x syringe (50 mL)
- 5 x tube with flow resistor
|
130-041-202 |
LS Column Adapter |
1 piece |
130-090-544 |
LS Column Adapter Kit |
- LS Column Adapter (130-090-544)
- LS Column plus tubes (130-041-306)
|
130-041-408 |
MACS 15 mL Tube Rack |
1 rack |
130-091-052 |
MACS Acrylic Tube Rack |
1 rack |
130-041-406 |
MACS Column Extensions |
100 pieces |
130-041-419 |
MACS MultiStand |
1 piece |
130-042-303 |
MS Column Adapter |
1 piece |
130-090-543 |
MS column Adapter Kit |
- MS Column Adapter (130-090-543)
- MS Column plus tubes (130-041-301)
|
130-041-401 |
OctoMACS Acrylic Tube Rack |
1 rack |
130-090-448 |
Separators
 |
- 最多可同時使用8個樣本
- 適用於大量的細胞分選
- 需使用專用的columns
|
Products: |
Capacity/Content/Components |
Order No. |
MiniMACS Starting Kit |
- MiniMACS Separator (130-042-102)
- MACS MultiStand (130-042-303)
- MS Columns (130-042-201)
- One MACS Cell Separation Reagent of Choice (includes only reagents with a capacity of up to 2x109 total cells)
|
130-090-312 |
MiniMACS Separator |
|
130-042-102 |
OctoMACS Starting Kit |
- OctoMACS Separator (130-042-109)
- MACS MultiStand (130-042-303)
- MS Columns (130-042-201)
- OctoMACS Acrylic Tube Rack (130-090-448)
- One MACS Cell Separation Reagent of Choice (includes only reagents with a capacity of up to 2x109 total cells)
|
130-042-108 |
OctoMACS Separator |
|
130-042-109 |
MidiMACS Starting Kit (LS) |
- MidiMACS Separator (130-042-302)
- MACS MultiStand (130-042-303)
- LS Columns (130-042-401)
- One MACS Cell Separation Reagent of choice (includes only reagents with a capacity of up to 2×109 total cells)
|
130-042-301 |
MidiMACS Starting Kit (LD) |
- MidiMACS Separator (130-042-302)
- MACS MultiStand (130-042-303)
- LD Columns (130-042-901)
- One MACS Cell Separation Reagent of choice (includes only reagents with a capacity of up to 2×109 total cells)
|
130-090-329 |
MidiMACS Separator |
|
130-042-302 |
QuadroMACS Starting Kit (LS) |
- QuadroMACS Separator (130-090-976)
- MACS MultiStand (130-042-303)
- LS Columns (130-042-401)
- MACS 15 mL Tube Rack (130-091-052)
- One MACS Cell Separation Reagent of choice (includes only reagents with a capacity of up to 2×109 total cells)
|
130-091-051 |
QuadroMACS Starting Kit (LD) |
- QuadroMACS Separator (130-090-976)
- MACS MultiStand (130-042-303)
- MACS 15 mL Tube Rack (130-091-052)
- LD Columns (130-042-901)
- One MACS Cell Separation Reagent of choice (includes only reagents with a capacity of up to 2×109 total cells)
|
130-092-857 |
QuadroMACS Separator |
|
130-090-976 |
Mini & MidiMACS Starting Kit
(MS, LS) |
- MiniMACS Separator (130-042-102)
- MidiMACS Separator (130-042-302)
- MACS MultiStand (130-042-303)
- MS Columns (130-042-201)
- LS Columns (130-042-401)
- One MACS Cell Separation Reagent of choice (includes only reagents with a capacity of up to 2×109 total cells)
|
130-042-501 |
Mini & MidiMACS Starting Kit
(MS, LD) |
- MiniMACS Separator (130-042-102)
- MidiMACS Separator (130-042-302)
- MACS MultiStand (130-042-303)
- MS Columns (130-042-201)
- LD Columns (130-042-901)
- One MACS Cell Separation Reagent of choice (includes only reagents with a capacity of up to 2×109 total cells)
|
130-091-632 |
VarioMACS Separator |
|
130-090-282 |
SuperMACS II Separator |
|
130-044-104 |
MACSiMAG Separator |
- MACSiMAG Separator
- Tube rack for tubes from 0.5 mL to 5 mL in size
|
130-092-168 |
Whole Blood MicroBeads
可直接從全血或骨髓樣本中分選出目標細胞,不需經過密度梯度離心或紅血球裂解的步驟,一次可使用0.25-15 mL的全血樣本,需使用專用的Whole Blood Column進行手動分選。可結合autoMACS Pro Separator或MultiMACS Cell24 separator,達到自動化分選細胞之目的,純化出的細胞可適用在流式細胞儀分析、分生實驗與細胞功能性測試。
 |
- 無密度梯度離心
- 不需紅血球裂解步驟
- 具有標準化的步驟
- 純度高
|
Products: |
Capacity/Content/Components |
Order No. |
Whole Blood CD3 MicroBeads, human |
for 40 mL whole blood |
130–090–874 |
Whole Blood CD4 MicroBeads, human |
for 40 mL whole blood |
130–090–877 |
Whole Blood CD8 MicroBeads, human |
for 40 mL whole blood |
130–090–878 |
Whole Blood and Bone Marrow CD138 MicroBeads, human |
for 40 mL whole blood |
130–105–961 |
Whole Blood Anti–ROR1 MicroBead Kit, human |
for 40 mL whole blood |
130–105–751 |
Whole Blood CD19 MicroBeads, human |
for 40 mL whole blood |
130–090–880 |
Whole Blood CD138 MicroBeads, human |
for 40 mL whole blood |
130–093–062 |
Whole Blood CD15 MicroBeads, human |
for 40 mL whole blood |
130–091–058 |
Whole Blood CD33 MicroBeads, human |
for 40 mL whole blood |
130–094–773 |
Whole Blood CD66b MicroBeads, human |
for 40 mL whole blood |
130–104–913 |
Whole Blood CD326 (EpCAM) MicroBeads, human |
for 40 mL whole blood |
130–109–827 |
Whole Blood CD326 (EpCAM) MicroBeads, human |
for 200 mL whole blood |
130–109–828 |
Whole Blood CD56 MicroBeads, human |
for 40 mL whole blood |
130–090–875 |
Whole Blood CD45 MicroBeads, human |
for 40 mL whole blood |
130–090–872 |
Whole Blood CD14 MicroBeads, human |
for 40 mL whole blood |
130–090–879 |
Whole Blood Column Kit |
10 x Whole Blood Columns
Whole Blood Column Elution Buffer |
130-093-545 |
Whole Blood MidiMACS Starting Kit |
- MidiMACS Separator (130-042-302)
- MACS MultiStand (130-042-303)
- MACS 15 mL Tube Rack (130-091-052)
- Whole Blood Column Kit (130-093-545)
- One vial of Whole Blood MicroBeads of choice
|
130-098-240 |
Whole Blood QuadroMACS Starting Kit |
- QuadroMACS Separator (130-042-976)
- MACS MultiStand (130-042-303)
- MACS 15 mL Tube Rack (130-091-052)
- Whole Blood Column Kit (130-093-545)
- One vial of Whole Blood MicroBeads of choice
|
130-098-242 |
Buffy Coat MicroBeads
由添加抗凝血的全血樣本經過低速血袋離心後分離出富含白血球與血小板的Buffy coat,Miltenyi Biotec推出的Buffy Coat MicroBeads能夠直接從Buffy Coat之中完整且溫和的分選出目標細胞,不需經過細胞計數與清洗等步驟,直接使用在Whole Blood Column進行目標細胞的手動分選,另可搭配MultiMACS Cell24 separator達到半自動化且標準化的細胞分選流程。
 |
- 無密度梯度離心
- 不需紅血球裂解
- 只需30分鐘即能分選出目標細胞
- 一套試劑適用於整個Buffy Coat (~80 mL)
|
Products |
Capacity/Content/Components |
Order No. |
Buffy Coat CD4 MicroBead Kit, human |
for 1 buffy coat
- 4 mL Buffy Coat CD4 MicroBeads
- 12×Whole Blood Columns
- Whole Blood Column Elution Buffer
|
130–114–980 |
Buffy Coat CD8 MicroBead Kit, human |
for 1 buffy coat
- 4 mL Buffy Coat CD8 MicroBeads
- 12×Whole Blood Columns
- Whole Blood Column Elution Buffer
|
130–114–978 |
Buffy Coat CD14 MicroBead Kit, human |
for 1 buffy coat
- 4 mL Buffy Coat CD14 MicroBeads
- 12×Whole Blood Columns
- Whole Blood Column Elution Buffer
|
130–114–976 |
Buffy Coat CD19 MicroBead Kit, human |
for 1 buffy coat
- 4 mL Buffy Coat CD19 MicroBeads
- 12×Whole Blood Columns
- Whole Blood Column Elution Buffer
|
130–114–974 |
Buffy Coat CD56 MicroBead Kit, human |
for 1 buffy coat
- 4 mL Buffy Coat CD56 MicroBeads
- 12×Whole Blood Columns
- Whole Blood Column Elution Buffer
|
130–114–963 |
REAlease Microbeads
REAlease Technology只需要簡單幾個步驟,就能夠在磁性分選之後移除細胞上標記的microbead與抗體。這個技術是利用重組抗體片段來代替完整的抗體進行細胞標記,單個重組抗體片段對於細胞表面抗原具有低親合力,可是將多個重組抗體片段聚合成為複合物 (REAlease Complex),就能以高親合力結合,並且透過間接的標記方式,進一步利用REAlease microbeads (anti-biotin) 結合到REAlease Complex (biotin),就能透過column與separator將目標細胞分選出。在細胞分選後添加REAlease Release Reagent使重組抗體片段單體化並自發性解離來移除細胞上標記的抗體,可獲得Label-free的細胞。
全新的磁性分選技術 |
 |
1. 間接的標記方式
細胞 + REAlease complex + REAlease microbead |
 |
3. 沖堤目標細胞
利用REAlease Bead Release Reagent將microbead移除 |
2. 磁性分選
目標細胞留在管柱內 |
4. 移除抗體標記
添加REAlease Release Reagent促進重組抗體片段的單體化與自發性解離
|
5. 獲得不帶有任何標記的目標細胞 |
 |
 |
- 不帶有microbead的細胞適合進行第二次分選
- 不帶有標記的細胞適用於任何的下游實驗
- 使用重組抗體片段給您可重複的實驗結果
|
roducts |
Capacity/Content/Components |
Order No. |
REAlease CD3 MicroBead Kit, human |
for 1×109 total cells |
130-117-038 |
REAlease CD4 MicroBead Kit, human |
for 1×109 total cells |
130-117-037 |
REAlease CD8 MicroBead Kit, human |
for 1×109 total cells |
130-117-036 |
REAlease CD19 MicroBead Kit, human |
for 1×109 total cells |
130-117-034 |
REAlease CD56 MicroBead Kit, human |
for 1×109 total cells |
130-117-033 |
MACSprep HLA cell Isolation Kit
Complement dependent cytotoxicity的相關研究中,需要分離出特定的HLA白血球,分離的過程非常複雜且容易有其他的細胞汙染。MACSprep HLA cell Isolation Kit提供快速和簡單的分選過程,並且取得純度高的B與T細胞。需搭配MACSxpress Separator一起使用。
 |
- 快速且非接觸性的分選出目標細胞
- 簡單的步驟,只需要20分鐘
- 無密度梯度離心
|
Products: |
Capacity/Content/Components |
Order No. |
MACSprep HLA B cell Isolation Kit, human |
for 5 x 5 mL whole blood |
130-110-130 |
MACSprep HLA T cell Isolation Kit, human |
for 5 x 5 mL whole blood |
130-110-128 |
MACSprep HLA B/T cell Isolation Kit, human |
for 5 x 5 mL whole blood |
130-110-129 |
MACSprep HLA Starting Kit MACSiMAG |
- MACSmix Tube Rotator (130-090-753)
- MACSiMAG Separator (130-092-168)
- One MACSprep HLA Cell Isolation Kit of choice
|
130-111-748 |
MACSprep HLA Starting Kit MACSxpress |
- MACSmix Tube Rotator (130-090-753)
- 2 x MACSxpress Separator (130-098-308)
- One MACSprep HLA Cell Isolation Kit of choice
|
130-111-749 |
MACSxpress
MACSxpress Technology是一個創新的細胞分離技術,可直接從大量的人類全血樣本中分選出目標細胞,無密度梯度離心,在快速、簡單且溫和的實驗步驟即可完成,一次可使用2 - 30 mL的全血樣本。使用MACSprep HLA cell Isolation Kit可以快速便捷的分選出B或T細胞,以利進行complement dependent cytotoxicity相關研究。
 |
- 20分鐘完成白血球分選
- 無密度梯度離心
- Untouched isolation
- 最多使用30 mL的全血樣本
|
|
Products: |
Capacity/Content/Components |
Order No. |
MACSxpress B Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–098–190 |
MACSxpress B–CLL Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–104–445 |
MACSxpress CD4 Memory T Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–106–770 |
MACSxpress CD4 T Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–098–195 |
MACSxpress CD8 T Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–098–194 |
MACSxpress Eosinophil Isolation Kit, human |
for 3×30 mL whole blood |
130–104–446 |
MACSxpress Erythrocyte Depletion Kit, human |
1 kit |
130–098–196 |
MACSxpress Naive B Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–098–186 |
MACSxpress Neutrophil Isolation Kit, human |
for 5×8 mL whole blood |
130–104–434 |
MACSxpress NK Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–098–185 |
MACSxpress Pan T Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–098–193 |
MACSxpress Pan B Cell Isolation Kit, human |
for 3×30 mL whole blood |
130–104–444 |
MACSxpress Treg Isolation Kit, human |
for 3×30 mL whole blood |
130–109–557 |
MACSxpress Separator |
1 unit |
130-098-308 |
MACSxpress Starting Kit |
- MACSxpress Separator (130-098-308)
- MACSmix Tube Rotator (130-090-753)
- One MACSxpress Isolation Kit of choice
|
130-101-319 |
Automated cell separation 全自動細胞分選儀器
面對龐大的樣本數,需要同時進行細胞分選,autoMACS Pro Separator與MultiMACS Cell24 separator可達成您的要求。Miltenyi Biotec提供全自動化的儀器且標準化的實驗流程,靈活運用您的實驗時間。如有更加快速的細胞分選、高產量的需求,Miltenyi Biotec與Tecan® Group Ltd. 合作推出liquid handling platforms,完全的支援MultiMACS Cell24 separator處理平台上的樣本,並有軟體監控整個細胞分選過程,可銜接MACSQuant Analyzer進行流式細胞儀分析。全自動化的儀器皆可使用在多個物種不同的單細胞懸浮液與人類全血樣本。
autoMACS Pro Separator
autoMACS Pro Separator是屬於benchtop的全自動化儀器,可放置於實驗桌或無菌操作檯內使用,適用於樣本數龐大且快速的細胞分選實驗,並有軟體監控整個流體系統、Columns狀態與直觀的操縱介面。
 |
- 標準化的實驗流程
- 全自動的細胞標記與分選過程,節省實驗時間
- 快速且溫和的分選不同種類的細胞樣本
- Walk-away fashion
|
|
Products: |
Capacity/Content/Components |
Order No. |
autoMACS Pro Separator – Starter Kit |
- autoMACS Pro Separator Instrument
- MACS MiniSampler
- MACS Reagent Rack 4 (130–094–574)
- Chill 5, 15, 50 Rack Set (130–097–038)
- barcode reader
- software
- autoMACS® Columns (130–021–101)
- 4×column substitute
- autoMACS Pro Buffer Combination
- User manual
- short instructions
- one–year warranty
|
130–092–545 |
autoMACS Bottle Extension Set |
- 3×color–coded sensor cable extension
- 4×color–coded tube extension
|
130–090–334 |
autoMACS Pro Angle Connector Set |
1 piece |
130–093–245 |
autoMACS Pro Autolabeling Upgrade Kit |
|
130–094–573 |
autoMACS Pro Laminar Hood Plate |
1 piece |
130–093–246 |
autoMACS Pro Protection Cover |
1 piece |
130–093–532 |
autoMACS® Columns |
5×2 columns |
130–021–101 |
Chill 5 Rack |
1 rack |
130–092–951 |
Chill 5 Racks |
3 racks |
130–097–041 |
Chill 5, 15, 50 Rack Set |
- Chill 5 Rack (130–092–951)
- Chill 15 Rack (130–092–952)
- Chill 50 Rack (130–092–953)
|
130–097–038 |
Chill 15 Rack |
1 rack |
130–092–952 |
Chill 15 Racks |
3 racks |
130–097–036 |
Chill 50 Rack |
1 rack |
130–092–953 |
Chill 50 Racks |
3 racks |
130–097–037 |
Chill 96 Rack |
1 rack |
130–094–459 |
Air–Filter Extension Set |
1 set |
130–091–339 |
MultiMACS Cell24 separator
彈性的選擇使用不同數量的樣本,最多同時使用24個樣本進行實驗,結合MACS microBeads與MACS Isolation Kits,能廣泛的應用在任何細胞種類與不同物種的細胞分選實驗,是屬於半自動化的實驗流程,可得到標準化且再現性高的實驗結果。能完全搭配Tecan' s Freedom EVO® liquid handling platforms達到全自動化的目的。
 |
- 同時處理多數的樣本
- 具有簡易的操作性與觸控螢幕
- 能夠使用單細胞懸浮液或人類全血樣本
|
|
Products: |
Capacity/Content/Components |
Order No. |
MultiMACS Cell24 Separator Plus |
- MultiMACS Cell24 Separator
- MACS Elution Station
- Elution Chamber Base
- Elution Chamber Lid I
- Elution Chamber Lid II
- Multi–24 Column Block (130–095–692)
- 2×24–well Deep Well Plate
- Single–Column Adapter
- MultiMACS 5 mL Tube Rack (130–095–331)
- Column Blank Holder and 9 Column Blanks
- Tubing
- Power cord
- Null modem cable
- High power CAN cable
|
130–098–637 |
Multi–24 Column Block |
- Multi–24 Column Block
- 24–well Deep Well Plate
|
130–095–692 |
Multi–24 Column Blocks |
- 8×Multi–24 Column Block
- 8×24–well Deep Well Plate
|
130–095–691 |
MultiMACS 5 mL Tube Rack |
1 rack |
130–095–331 |
MultiMACS 12x Single–Column Adapter LS |
- 1× Single‑Column Adapter
- 12× Column Blanks
|
130–108–816 |
24–well Deep Well Plates |
4 pieces |
130–110–500 |
Single–well Deep Well Plates |
2×2 pieces |
130–114–966 |
Tecan' s Freedom EVO® liquid handling platforms
Tecan公司是液體處理系統的頂尖供應商,與Miltenyi Biotec正在共同改革多方面且全自動的細胞學工作流程,並推出Tecan' s Freedom EVO® liquid handling platforms,能夠完全的支援MultiMACS Cell24 separator進行細胞分選實驗,提供一個精確控制液體流向的全自動樣本處理平台,並且具有軟體讓您監控整個細胞分選過程與儀器狀態,更能夠銜接流式細胞儀進行實驗分析。
 |
- 全自動化的儀器
- 同時處理多數樣本及複雜的流程
- 靈活的滿足您不同的實驗需求
|
Cell separator buffer 細胞分選專用緩衝液
MACS 的細胞分選專用緩衝液都是經過最適化與嚴格的品質控管,具有高品質的成分與無菌過濾,確保您得到優異的細胞分選效率。
AstroMACS Separation buffer專門用於星狀膠細胞的分選實驗。
由於神經細胞非常脆弱,往往在成鼠腦組織解離過程中產生大量的死細胞,進而影響到星狀膠細胞的培養實驗。AstroMACS separation buffer能夠在細胞分選過程有效率的移除死細胞干擾,可搭配AstroMACS medium達到最佳的星狀膠細胞培養。
 |
- 最佳化: 得到最好的細胞分選結果
- 能直接使用,節省實驗時間
- 皆可用在手動與自動化的細胞分選實驗
|
Products: |
Capacity/Content/Components |
Order No. |
autoMACS Pro Washing Solution |
6 x 1.5 L |
130-092-987 |
autoMACS Rinsing Solution |
6 x 1.45 L |
130-091-222 |
autoMACS Running – MACS Separation Buffer |
6 x 1.5 L |
130-091-221 |
MACS BSA Stock Solution |
6 x 75 mL |
130-091-376 |
AstroMACS Separation Buffer |
100 mL |
130-117-336 |
Selection Strategy 分選策略
MACS Technology 細胞分選的黃金標準
細胞分選只需30分鐘 !
 |
- Nano-sized 直徑 50 nm
- 生物可分解、無毒性
- 不影響細胞活化與功能
|
Positive Selection 取得有標記的目標細胞
可最大限度的減少對目標細胞的不良影響。
 |
磁性標記特定的目標細胞,被標記的目標細胞停留在管柱內,未標記的細胞被沖洗後移除,隨後將管柱取下,洗脫出目標細胞後並收集。 |
 |
使用MACS Separator進行手動分選
QuadroMACS (左) 和 MidiMACS (右) Separators |
系列產品
- MACS MicroBeads
- StraightFrom™ MicroBeads
- REAlease™ MicroBead
Untouched Selection 取得無標記的目標細胞
透過磁性標記大部分不想要的細胞,為了使特定的目標細胞保持在untouched form,不會對目標細胞產生壓力。
 |
未標記的目標細胞被洗脫後並收集,不想要的細胞仍停留在管柱內。 |
系列產品
Cell Isolation Kits 含有特定濃度的抗體雞尾酒和用於間接磁性標記的MACS MicroBeads
- MACS Cell Isolation Kits
- MACSxpress Cell Isolation Kit
取得無標記的腫瘤細胞
從解離的組織中移除非腫瘤細胞對於取得純化的腫瘤細胞是必要的。在全面性的篩選了原發性腫瘤、細胞株、和健康組織後,Miltenyi Biotec找出了識別腫瘤微環境內細胞的抗體組合,這些抗體不會識別腫瘤細胞。使用與這些抗體偶聯的MicroBeads,可在20分鐘內移除>95%的非腫瘤細胞。
預先純化的TIL可改善分析結果
 |
使用Tumor Cell Isolation Kit, human,從混雜的人類細胞樣本中分離
EpCAM陽性人乳腺癌細胞。隨後將分選前與純化後的細胞培養七天後
進行染色。 |
(紅色)Vimentin蛋白,標記纖維母細胞
(綠色)EpCAM,標記腫瘤細胞
(藍色)DAPI,標記細胞核
Products: |
Capacity/Content/Components |
Order No. |
Tumor Cell Isolation Kit, human |
for 5×10⁸ total cells, up to 50 separations |
130-108-339 |
Tumor Cell Isolation Kit, mouse |
for 5×10⁸ total cells, up to 50 separations |
130-110-187 |
Tumor-Associated Fibroblast Isolation Kit, mouse
從解離的小鼠腫瘤中分離組織來源的腫瘤相關纖維母細胞 |
for 5×10⁸ total cells, up to 50 separations |
130-116-474 |
Mouse Cell Depletion Kit
從解離的小鼠異種移植腫瘤中純化出無標記的人類細胞 |
for 1×10⁹ total cells, 100 separations |
130-104-694 |