Lymphocyte Separation Medium |
Cell isolation |
Cell separation reagents
細胞分選試劑 ![]() |
‧ T cell ‧ B cell ‧ NK cell ‧ Dendritic cell ‧ Monocytes and Macrophage ‧ Granulocytes and Myeloid cells ‧ Cytokine-producing cells ‧ Endothlial cells ‧ Tumor cells ‧ Cancer stem cells ‧ Hematopoietic stem cells ‧ Mesenchymal stem cells ‧ ES and iPS cells ‧ Neural cells ‧ Cardiac cells ‧ Any cell types ‧ Various cell types |
Manual cell separation
手動細胞分選設備 ![]() |
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Automated cell separation
全自動細胞分選儀器 ![]() |
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Cell separation buffers
細胞分選專用緩衝液 ![]() |
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Selection Strategy
分選策略 ![]() |
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起始 材料 |
Tissue single cell suspension or PBMC |
Whole blood or Bone marrow |
Buffy coat | Whole blood or Spleen | Whole blood |
試劑 套組 |
Cell separation reagents (microbeads) | Whole blood microbeads | Buffy coat microbeads | MACSprep HLA isolation kits | MACSxpress |
手動細胞分選 | MACS columns | Whole blood column | Whole blood column | MACSxpress | MACSxpress |
自動細胞分選 | autoMACS pro or MultiMACS Cell24 |
autoMACS pro or MultiMACS Cell24 |
MultiMACS Cell24 | X | X |
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Microbeads為超順磁性的奈米粒子(~50 nm) 生物可分解且沒有毒性 適用於不同物種及任何細胞種類 可應用在基礎與臨床研究 |
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Products: | Capacity/Content/Components | Order No. |
CS columns |
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130-041-305 |
D columns |
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130-041-201 |
Large Cell Columns |
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130-042-202 |
LD Columns |
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130-042-901 |
LS Columns |
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130-042-401 |
LS Columns plus tubes |
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130-041-306 |
MS Columns |
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130-042-201 |
MS Columns plus tubes |
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130-041-301 |
Whole Blood Column Kit |
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130-093-545 |
XS Columns |
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130-041-202 |
LS Column Adapter | 1 piece | 130-090-544 |
LS Column Adapter Kit |
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130-041-408 |
MACS 15 mL Tube Rack | 1 rack | 130-091-052 |
MACS Acrylic Tube Rack | 1 rack | 130-041-406 |
MACS Column Extensions | 100 pieces | 130-041-419 |
MACS MultiStand | 1 piece | 130-042-303 |
MS Column Adapter | 1 piece | 130-090-543 |
MS column Adapter Kit |
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130-041-401 |
OctoMACS Acrylic Tube Rack | 1 rack | 130-090-448 |
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Products: | Capacity/Content/Components | Order No. |
MiniMACS Starting Kit |
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130-090-312 |
MiniMACS Separator |
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130-042-102 |
OctoMACS Starting Kit |
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130-042-108 |
OctoMACS Separator |
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130-042-109 |
MidiMACS Starting Kit (LS) |
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130-042-301 |
MidiMACS Starting Kit (LD) |
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130-090-329 |
MidiMACS Separator |
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130-042-302 |
QuadroMACS Starting Kit (LS) |
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130-091-051 |
QuadroMACS Starting Kit (LD) |
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130-092-857 |
QuadroMACS Separator |
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130-090-976 |
Mini & MidiMACS Starting Kit (MS, LS) |
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130-042-501 |
Mini & MidiMACS Starting Kit (MS, LD) |
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130-091-632 |
VarioMACS Separator |
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130-090-282 |
SuperMACS II Separator |
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130-044-104 |
MACSiMAG Separator |
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130-092-168 |
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Products: | Capacity/Content/Components | Order No. |
Whole Blood CD3 MicroBeads, human | for 40 mL whole blood | 130–090–874 |
Whole Blood CD4 MicroBeads, human | for 40 mL whole blood | 130–090–877 |
Whole Blood CD8 MicroBeads, human | for 40 mL whole blood | 130–090–878 |
Whole Blood and Bone Marrow CD138 MicroBeads, human | for 40 mL whole blood | 130–105–961 |
Whole Blood Anti–ROR1 MicroBead Kit, human | for 40 mL whole blood | 130–105–751 |
Whole Blood CD19 MicroBeads, human | for 40 mL whole blood | 130–090–880 |
Whole Blood CD138 MicroBeads, human | for 40 mL whole blood | 130–093–062 |
Whole Blood CD15 MicroBeads, human | for 40 mL whole blood | 130–091–058 |
Whole Blood CD33 MicroBeads, human | for 40 mL whole blood | 130–094–773 |
Whole Blood CD66b MicroBeads, human | for 40 mL whole blood | 130–104–913 |
Whole Blood CD326 (EpCAM) MicroBeads, human | for 40 mL whole blood | 130–109–827 |
Whole Blood CD326 (EpCAM) MicroBeads, human | for 200 mL whole blood | 130–109–828 |
Whole Blood CD56 MicroBeads, human | for 40 mL whole blood | 130–090–875 |
Whole Blood CD45 MicroBeads, human | for 40 mL whole blood | 130–090–872 |
Whole Blood CD14 MicroBeads, human | for 40 mL whole blood | 130–090–879 |
Whole Blood Column Kit | 10 x Whole Blood Columns Whole Blood Column Elution Buffer |
130-093-545 |
Whole Blood MidiMACS Starting Kit |
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130-098-240 |
Whole Blood QuadroMACS Starting Kit |
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130-098-242 |
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Products | Capacity/Content/Components | Order No. |
Buffy Coat CD4 MicroBead Kit, human | for 1 buffy coat
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130–114–980 |
Buffy Coat CD8 MicroBead Kit, human | for 1 buffy coat
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130–114–978 |
Buffy Coat CD14 MicroBead Kit, human | for 1 buffy coat
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130–114–976 |
Buffy Coat CD19 MicroBead Kit, human | for 1 buffy coat
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130–114–974 |
Buffy Coat CD56 MicroBead Kit, human | for 1 buffy coat
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130–114–963 |
全新的磁性分選技術 | |||
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1. 間接的標記方式 細胞 + REAlease complex + REAlease microbead |
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3. 沖堤目標細胞 利用REAlease Bead Release Reagent將microbead移除 |
2. 磁性分選 目標細胞留在管柱內 |
4. 移除抗體標記 添加REAlease Release Reagent促進重組抗體片段的單體化與自發性解離 |
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5. 獲得不帶有任何標記的目標細胞 | |||
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Products | Capacity/Content/Components | Order No. |
REAlease CD3 MicroBead Kit, human | for 1×109 total cells | 130-117-038 |
REAlease CD4 MicroBead Kit, human | for 1×109 total cells | 130-117-037 |
REAlease CD8 MicroBead Kit, human | for 1×109 total cells | 130-117-036 |
REAlease CD19 MicroBead Kit, human | for 1×109 total cells | 130-117-034 |
REAlease CD56 MicroBead Kit, human | for 1×109 total cells | 130-117-033 |
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Products: | Capacity/Content/Components | Order No. |
MACSprep HLA B cell Isolation Kit, human | for 5 x 5 mL whole blood | 130-110-130 |
MACSprep HLA T cell Isolation Kit, human | for 5 x 5 mL whole blood | 130-110-128 |
MACSprep HLA B/T cell Isolation Kit, human | for 5 x 5 mL whole blood | 130-110-129 |
MACSprep HLA Starting Kit MACSiMAG |
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130-111-748 |
MACSprep HLA Starting Kit MACSxpress |
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130-111-749 |
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Products: | Capacity/Content/Components | Order No. |
MACSxpress B Cell Isolation Kit, human | for 3×30 mL whole blood | 130–098–190 |
MACSxpress B–CLL Cell Isolation Kit, human | for 3×30 mL whole blood | 130–104–445 |
MACSxpress CD4 Memory T Cell Isolation Kit, human | for 3×30 mL whole blood | 130–106–770 |
MACSxpress CD4 T Cell Isolation Kit, human | for 3×30 mL whole blood | 130–098–195 |
MACSxpress CD8 T Cell Isolation Kit, human | for 3×30 mL whole blood | 130–098–194 |
MACSxpress Eosinophil Isolation Kit, human | for 3×30 mL whole blood | 130–104–446 |
MACSxpress Erythrocyte Depletion Kit, human | 1 kit | 130–098–196 |
MACSxpress Naive B Cell Isolation Kit, human | for 3×30 mL whole blood | 130–098–186 |
MACSxpress Neutrophil Isolation Kit, human | for 5×8 mL whole blood | 130–104–434 |
MACSxpress NK Cell Isolation Kit, human | for 3×30 mL whole blood | 130–098–185 |
MACSxpress Pan T Cell Isolation Kit, human | for 3×30 mL whole blood | 130–098–193 |
MACSxpress Pan B Cell Isolation Kit, human | for 3×30 mL whole blood | 130–104–444 |
MACSxpress Treg Isolation Kit, human | for 3×30 mL whole blood | 130–109–557 |
MACSxpress Separator | 1 unit | 130-098-308 |
MACSxpress Starting Kit |
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130-101-319 |
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Products: | Capacity/Content/Components | Order No. |
autoMACS Pro Separator – Starter Kit |
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130–092–545 |
autoMACS Bottle Extension Set |
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130–090–334 |
autoMACS Pro Angle Connector Set | 1 piece | 130–093–245 |
autoMACS Pro Autolabeling Upgrade Kit | 130–094–573 | |
autoMACS Pro Laminar Hood Plate | 1 piece | 130–093–246 |
autoMACS Pro Protection Cover | 1 piece | 130–093–532 |
autoMACS® Columns | 5×2 columns | 130–021–101 |
Chill 5 Rack | 1 rack | 130–092–951 |
Chill 5 Racks | 3 racks | 130–097–041 |
Chill 5, 15, 50 Rack Set |
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130–097–038 |
Chill 15 Rack | 1 rack | 130–092–952 |
Chill 15 Racks | 3 racks | 130–097–036 |
Chill 50 Rack | 1 rack | 130–092–953 |
Chill 50 Racks | 3 racks | 130–097–037 |
Chill 96 Rack | 1 rack | 130–094–459 |
Air–Filter Extension Set | 1 set | 130–091–339 |
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Products: | Capacity/Content/Components | Order No. |
MultiMACS Cell24 Separator Plus |
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130–098–637 |
Multi–24 Column Block |
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130–095–692 |
Multi–24 Column Blocks |
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130–095–691 |
MultiMACS 5 mL Tube Rack | 1 rack | 130–095–331 |
MultiMACS 12x Single–Column Adapter LS |
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130–108–816 |
24–well Deep Well Plates | 4 pieces | 130–110–500 |
Single–well Deep Well Plates | 2×2 pieces | 130–114–966 |
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Products: | Capacity/Content/Components | Order No. |
autoMACS Pro Washing Solution | 6 x 1.5 L | 130-092-987 |
autoMACS Rinsing Solution | 6 x 1.45 L | 130-091-222 |
autoMACS Running – MACS Separation Buffer | 6 x 1.5 L | 130-091-221 |
MACS BSA Stock Solution | 6 x 75 mL | 130-091-376 |
AstroMACS Separation Buffer | 100 mL | 130-117-336 |
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磁性標記特定的目標細胞,被標記的目標細胞停留在管柱內, 未標記的細胞被沖洗後移除,隨後將管柱取下,洗脫出目標細 胞後並收集。 |
使用MACS Separator進行手動分選 QuadroMACS (左) 和 MidiMACS (右) Separators |
未標記的目標細胞被洗脫後並收集, 不想要的細胞仍停留在管柱內。 |
使用Tumor Cell Isolation Kit, human, 從混雜的人類細胞樣本中分離EpCAM陽 性人乳腺癌細胞。隨後將分選前與純化後 的細胞培養七天後進行染色。 |
Products: | Capacity/Content/Components | Order No. |
Tumor Cell Isolation Kit, human | for 5×10⁸ total cells, up to 50 separations | 130-108-339 |
Tumor Cell Isolation Kit, mouse | for 5×10⁸ total cells, up to 50 separations | 130-110-187 |
Tumor-Associated Fibroblast Isolation Kit, mouse 從解離的小鼠腫瘤中分離組織來源的腫瘤相關纖維母細胞 |
for 5×10⁸ total cells, up to 50 separations | 130-116-474 |
Mouse Cell Depletion Kit 從解離的小鼠異種移植腫瘤中純化出無標記的人類細胞 |
for 1×10⁹ total cells, 100 separations | 130-104-694 |