Protein Research

Western Blot

Pre-Cast Gel
 
Protein Ladder and Loading dye
 
Protein Stain
 

Quick Coomassie Stain | 快速蛋白質膠體Coomassie藍染劑
  • 快速、安全、靈敏度高、無毒的Coomassie G-250 染劑
  • 15分鐘完成染色,無需退染
  • 無需使用有機溶劑e.g., methanol 或 acetic acid 
  • 與下游分析MS兼容
  • 最低可偵測5 ng 蛋白質

QC Stain 的靈敏度優於它牌藍染劑​

 
Cat. No. Product Size
QC-STAIN-1L Quick Commassie Stain 1 L
 

Genecopoeia

eLuminol™ Protein Gel Stain | 蛋白質膠體螢光染劑 
  • 具有寬廣的線性偵測範圍(至少三個log)
  • 不需固定步驟,可簡單快速的進行膠體染色
  • 與下游實驗相容,如質譜分析及定序
  • 高靈敏度,可偵測約0.5 ng的蛋白質
 
Cat. No. Product Size
P003A eLuminol™ Protein Gel Stain (1,000X) 500 µl
P003B eLuminol™ Protein Gel Stain (1,000X) 1 mL
 
Protein Gel Stain Selection Guide
  Quick Commassie Stain eLuminol™ Protein
Gel Stain
Sensitivity 5 ng 0.5 ng
Ease of use Ready to use Supplied as stock solution
Time required 15 min (standard)
30 min (microwave)
90 min (standard)
30 min (microwave)
Detection method colorimetric fluorescent
MS spec. compatible Yes Yes
 

Buffers
       Bis-Acrylamide, 2% Solution (GoldBio)
       Bis-Acrylamide 250 g (GoldBio)
       Tris-Glycine (TG) Buffer pH 8.3 (Medicago)
       Tris-Glycine-SDS (TG-SDS) Buffer pH 8.3 (Medicago)
       Tris Buffer (Tris-HCl) pH 7.4, pH 8.0 and pH 8.3 (Medicago)

Reagents
       Glycerol (Glycerin) (Medicago)
       Bromophenol Blue Free Acid, ACS Grade (GoldBio)

Detergents
       Tween™ 20 (Medicago)
       BRIJ-35 (GoldBio)
       n-Dodecyl-Β-D-maltoside (DDM) (GoldBio)
       Sodium Dodecyl Sulphate Reagent (SDS) (Medicago)
       CHAPS (GoldBio)

Reducing Agents
       DTE (Dithioerythritol) (GoldBio)
       DTT (Dithiothreitol) (> 99% pure) Protease free (GoldBio)
       TCEP, 1 g (ProteinArk)
       TCEP, 10 g (ProteinArk)
       TCEP-HCl (GoldBio)

Protein Ladder (SMOBIO)

SMOBIO  
PM1500 All Blue, 10-180 kDa
    
SMOBIO
PM1600 All Blue, 10-180 kDa
    
SMOBIO
PM1700 All Blue, 10-240 kDa
 
SMOBIO
PM2400 Pink Blue, 10-175 kDa
      
SMOBIO
PM2500 3-color, 10-180 kDa
 
SMOBIO
PM2600 3-color, 10-245 kDa
SMOBIO
PM2700 3-color, 5-245 kDa
SMOBIO
PM5000 3-color, 10-180 kDa
SMOBIO
PM5100 3-color, 10-245 kDa
SMOBIO
PM5200 3-color, 5-245 kDa


Gel Stain
       Real-time Stain (ProteinArk)
       PS1000/PS1001 FluoroStain Protein Fluorescent Staining Dye (Red, 1000x) (SMOBIO)
       PS2000 FluoroStain Protein Fluorescent Staining Dye (Gold, 100x) (SMOBIO)
       Sapphire Coomassie Powder Kit (GoldBio) 
       Coomassie Brilliant Blue G-250 (GoldBio)
       Coomassie Brilliant Blue G-250 (Genlantis)
       Coomassie Brilliant Blue R-250 (GoldBio)
       Coomassie Brilliant Blue R-250 (Genlantis)
 
Stripping Buffer
能夠去除membrane上的反應液、一抗和二抗,處理過的膜可以用原來的抗體或其他的抗體,重複進行檢測。


Stripping Buffer (Candor)
  • 即開即用,不需加熱步驟。在室溫下溫和震盪30-60分鐘即可完成抗體移除
  • 成分不含有β-Mercaptoethanol 或DTT,無臭味產生


BlotFresh™ Western Blot Stripping Reagent (Ver. II) (SignaGen)
  • 快速,不需加熱步驟!在室溫下震盪5-15分鐘即可完成抗體移除
  • 成分不含有β-Mercaptoethanol或DTT,無臭味產生
  • PVDF或NC memberane皆適用
  • 適合移除抗體和Mass Spectrometry應用

BlotFresh™ Plus Western Blot Stripping Reagent (SignaGen)
  • 快速,不需加熱步驟!在室溫下震盪5-15分鐘即可完成抗體移除
  • 成分不含有β-Mercaptoethanol或DTT,無臭味產生
  • PVDF或NC memberane皆適用
  • 適合移除高親和性抗體Mass Spectrometry應用。室溫下穩定保存。保存期限12個月​
 

Stabilizers

HRP-Protector (Candor)
LowCross-HRP (Candor)

Stain

Ponceau S (GoldBio)
Ponceau S Solution 1 mg/mL (GoldBio)
Ponceau S solution 0.1% (w/v) Ponceau S in 5% acetic acid. (Boston BioProsucts)

Substrate

LumiGOLD™ ECL Western Blotting Detection Kit (Ver. II) (SignaGen) 
ReadyTector® Chemiluminescent Substrate (ECL) (Candor)
BCIP/NBT Substrate, 100 ml (for the detection of alkaline phosphatase in membrane applications)(Cygnus)
BCIP/NBT Substrate, 1000 ml (for the detection of alkaline phosphatase in membrane applications)(Cygnus)
TMB Substrate, for Western Blot, 100 ml (Cygnus)
TMB Substrate, for Western Blot, 1000 ml (Cygnus)
TMB dihydrochloride, Powder (GoldBio)
TMB, free base, Powder (GoldBio)
 
ECL


UltraScence Western Substrate是以發光胺(luminol)為基底的強化型化學冷光受質,可和接有HRP的二級抗體
反應且靈敏度高,並且具有出色的長時間訊號持續度,無論在數位影像或膠片影像都不會有訊號流失的情況。

三種不同靈敏度的產品,分別為
  • UltraScence Pico Plus Western Substrate
    • 靈敏度:low picogram to high femtogram
    • 為入門級試劑,適合使用於偵測高表現量的蛋白質訊號
  • UltraScence Pico Ultra Western Substrate
    • 靈敏度:low picogram to mid femtogram
    • 靈敏度為UltraScence Pico Plus的30倍,適合使用於偵測較低表現量的蛋白質訊號
  • UltraScence Femto Western Substrate
    • 靈敏度:mid femtogram to low femtogram
    • 超高靈敏度,適合使用於偵測極低表現量的蛋白質訊號

  產品效能比較  
UltraScence Pico Plus Western Substrate
免疫印跡訊號強度比較 化學冷光訊號比較
  • 蛋白質樣品:HeLa whole cell lysates
  • Western substrate:4 ml
  • Imaging:UVP BIO SPECTRUM-AC digital imaging system
比較不同廠牌冷光受質與HRP反應後的冷光訊號強度 :
 
UltraScence Femto Western Substrate
免疫印跡訊號強度比 化學冷光訊號比較
  • 蛋白質樣品:mock RD cell lysates
  • Western substrate:4 ml
  • Imaging:Chemlux SPX-600 Series digital imaging system
 
  廠牌相似靈敏度對照表  
Bio-HeliX產品   靈敏度 其他廠牌相似靈敏度產品
UltraScence
Pico Plus
Western Substrate
low picogram to
high femtogram
Thermo Scientfic™ Pierce™ ECL Substrate
Thermo Scientfic™ SuperSignal™ West Pico PLUS
CYANAGEN™ WESTAR™ ETA C 2.0
Advansta™ WesternBright™ ECL
Expedeon™ LumiBlue ECL Extra
UltraScence
Pico Ultra
Western Substrate
low picogram to
mid femtogram
Millipore™ Immobilon™ Western Substrate
FUJIFILM™ Wako™ ImmunoStar™ Zeta
GE Healthcare™ Amersham™ ECL™ Prime
Advansta™ WesternBright™ Quantum™
Expedeon™ LumiBlue™ ECL Extended
CYANAGEN™ WESTAR™ ETA C ULTRA 2.0
Thermo Scientfic™ SuperSignal™ West DURA
UltraScence
Femto
Western Substrate
mid femtogram to
low femtogram
FUJIFILM™ Wako™ ImmunoStar™ LD
GeneTex™ Trident™ femto
Thermo Scientfic™ SuperSignal™ West Femto
Advansta™ WesternBright™ Sirius™
Expedeon™ LumiBlue™ ECL Extreme
CYANAGEN™ WESTAR™ SUPERNOVA™
GE Healthcare™ SELECT