Western Blot
| Pre-Cast Gel |
| Protein Ladder and Loading dye |
| Protein Stain |
Quick Coomassie Stain | 快速蛋白質膠體Coomassie藍染劑
- 快速、安全、靈敏度高、無毒的Coomassie G-250 染劑
- 15分鐘完成染色，無需退染
- 無需使用有機溶劑e.g., methanol 或 acetic acid
- 與下游分析MS兼容
- 最低可偵測5 ng 蛋白質
QC Stain 的靈敏度優於它牌藍染劑
| Cat. No. | Product | Size |
| QC-STAIN-1L | Quick Commassie Stain | 1 L |
eLuminol™ Protein Gel Stain | 蛋白質膠體螢光染劑
- 具有寬廣的線性偵測範圍(至少三個log)
- 不需固定步驟，可簡單快速的進行膠體染色
- 與下游實驗相容，如質譜分析及定序
- 高靈敏度，可偵測約0.5 ng的蛋白質
| Cat. No. | Product | Size |
| P003A | eLuminol™ Protein Gel Stain (1,000X) | 500 µl |
| P003B | eLuminol™ Protein Gel Stain (1,000X) | 1 mL |
Protein Gel Stain Selection Guide
| Quick Commassie Stain | eLuminol™ Protein Gel Stain |
|
| Sensitivity | 5 ng | 0.5 ng |
| Ease of use | Ready to use | Supplied as stock solution |
| Time required | 15 min (standard) 30 min (microwave) |
90 min (standard) 30 min (microwave) |
| Detection method | colorimetric | fluorescent |
| MS spec. compatible | Yes | Yes |
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 |
Buffers
Bis-Acrylamide, 2% Solution (GoldBio)
Bis-Acrylamide 250 g (GoldBio)
Tris-Glycine (TG) Buffer pH 8.3 (Medicago)
Tris-Glycine-SDS (TG-SDS) Buffer pH 8.3 (Medicago)
Tris Buffer (Tris-HCl) pH 7.4, pH 8.0 and pH 8.3 (Medicago)
Reagents
Glycerol (Glycerin) (Medicago)
Bromophenol Blue Free Acid, ACS Grade (GoldBio)
Detergents
Tween™ 20 (Medicago)
BRIJ-35 (GoldBio)
n-Dodecyl-Β-D-maltoside (DDM) (GoldBio)
Sodium Dodecyl Sulphate Reagent (SDS) (Medicago)
CHAPS (GoldBio)
Reducing Agents
DTE (Dithioerythritol) (GoldBio)
DTT (Dithiothreitol) (> 99% pure) Protease free (GoldBio)
TCEP, 1 g (ProteinArk)
TCEP, 10 g (ProteinArk)
TCEP-HCl (GoldBio)
Protein Ladder (SMOBIO)
Gel Stain
Real-time Stain (ProteinArk)
PS1000/PS1001 FluoroStain Protein Fluorescent Staining Dye (Red, 1000x) (SMOBIO)
PS2000 FluoroStain Protein Fluorescent Staining Dye (Gold, 100x) (SMOBIO)
Sapphire Coomassie Powder Kit (GoldBio)
Coomassie Brilliant Blue G-250 (GoldBio)
Coomassie Brilliant Blue G-250 (Genlantis)
Coomassie Brilliant Blue R-250 (GoldBio)
Coomassie Brilliant Blue R-250 (Genlantis)
| Stripping Buffer |
Stripping Buffer (Candor)
- 即開即用，不需加熱步驟。在室溫下溫和震盪30-60分鐘即可完成抗體移除
- 成分不含有β-Mercaptoethanol 或DTT，無臭味產生
BlotFresh™ Western Blot Stripping Reagent (Ver. II) (SignaGen)
- 快速，不需加熱步驟！在室溫下震盪5-15分鐘即可完成抗體移除
- 成分不含有β-Mercaptoethanol或DTT，無臭味產生
- PVDF或NC memberane皆適用
- 適合移除抗體和Mass Spectrometry應用
BlotFresh™ Plus Western Blot Stripping Reagent (SignaGen)
- 快速，不需加熱步驟！在室溫下震盪5-15分鐘即可完成抗體移除
- 成分不含有β-Mercaptoethanol或DTT，無臭味產生
- PVDF或NC memberane皆適用
- 適合移除高親和性抗體Mass Spectrometry應用。室溫下穩定保存。保存期限12個月
Stabilizers
HRP-Protector (Candor)LowCross-HRP (Candor)
Stain
Ponceau S (GoldBio)Ponceau S Solution 1 mg/mL (GoldBio)
Ponceau S solution 0.1% (w/v) Ponceau S in 5% acetic acid. (Boston BioProsucts)
Substrate
LumiGOLD™ ECL Western Blotting Detection Kit (Ver. II) (SignaGen)ReadyTector® Chemiluminescent Substrate (ECL) (Candor)
BCIP/NBT Substrate, 100 ml (for the detection of alkaline phosphatase in membrane applications)(Cygnus)
BCIP/NBT Substrate, 1000 ml (for the detection of alkaline phosphatase in membrane applications)(Cygnus)
TMB Substrate, for Western Blot, 100 ml (Cygnus)
TMB Substrate, for Western Blot, 1000 ml (Cygnus)
TMB dihydrochloride, Powder (GoldBio)
TMB, free base, Powder (GoldBio)
| ECL |
UltraScence Western Substrate是以發光胺(luminol)為基底的強化型化學冷光受質，可和接有HRP的二級抗體
反應且靈敏度高，並且具有出色的長時間訊號持續度，無論在數位影像或膠片影像都不會有訊號流失的情況。
三種不同靈敏度的產品，分別為
- UltraScence Pico Plus Western Substrate
- 靈敏度：low picogram to high femtogram
- 為入門級試劑，適合使用於偵測高表現量的蛋白質訊號
- UltraScence Pico Ultra Western Substrate
- 靈敏度：low picogram to mid femtogram
- 靈敏度為UltraScence Pico Plus的30倍，適合使用於偵測較低表現量的蛋白質訊號
- UltraScence Femto Western Substrate
- 靈敏度：mid femtogram to low femtogram
- 超高靈敏度，適合使用於偵測極低表現量的蛋白質訊號
產品效能比較
| UltraScence Pico Plus Western Substrate | |
| 免疫印跡訊號強度比較 | 化學冷光訊號比較 |
|
比較不同廠牌冷光受質與HRP反應後的冷光訊號強度 : |
| UltraScence Femto Western Substrate | |
| 免疫印跡訊號強度比 | 化學冷光訊號比較 |
|
 |
廠牌相似靈敏度對照表
| Bio-HeliX產品 | 靈敏度 | 其他廠牌相似靈敏度產品 | |
| UltraScence Pico Plus Western Substrate |
 |
low picogram to high femtogram |
Thermo Scientfic™ Pierce™ ECL Substrate Thermo Scientfic™ SuperSignal™ West Pico PLUS CYANAGEN™ WESTAR™ ETA C 2.0 Advansta™ WesternBright™ ECL Expedeon™ LumiBlue ECL Extra |
| UltraScence Pico Ultra Western Substrate |
 |
low picogram to mid femtogram |
Millipore™ Immobilon™ Western Substrate FUJIFILM™ Wako™ ImmunoStar™ Zeta GE Healthcare™ Amersham™ ECL™ Prime Advansta™ WesternBright™ Quantum™ Expedeon™ LumiBlue™ ECL Extended CYANAGEN™ WESTAR™ ETA C ULTRA 2.0 Thermo Scientfic™ SuperSignal™ West DURA |
| UltraScence Femto Western Substrate |
 |
mid femtogram to low femtogram |
FUJIFILM™ Wako™ ImmunoStar™ LD GeneTex™ Trident™ femto Thermo Scientfic™ SuperSignal™ West Femto Advansta™ WesternBright™ Sirius™ Expedeon™ LumiBlue™ ECL Extreme CYANAGEN™ WESTAR™ SUPERNOVA™ GE Healthcare™ SELECT |