Cell Analysis

Cell Apoptosis Assay 細胞凋亡檢測

 
Annexin V Binding Assays
   
在細胞凋亡的早期,原本在細胞膜內側的磷脂酰絲氨酸(Phosphatidylserine, PS)會從內側翻轉到細胞膜外側。透過可與PS高親和力、特異性結合的螢光標定Annexin V,即可簡單、快速地檢測出細胞早期凋亡情況。
 
◆靈敏度高—使用Andy Fluor™ 標記,明亮的信號      
◆靈活性—具多種顏色螢光可選擇,有利於多重染色實驗
◆操作簡單—僅需30分鐘即可完成檢測
   
 
 
Specifications
Platform: Fluorescence Cytometer
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; eGFP: 475/509 nm

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Specifications
Platform: Fluorescence Cytometer
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; FITC: 495/520 nm

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Specifications
Platform: Fluorescence Cytometer
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; Andy Fluor™ 488: 495/520 nm

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Specifications
Platform: Fluorescence Cytometer, Fluorescence Microscopy
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; Andy Fluor™ 488: 495/520 nm; Hoechst 33324: 350/460 nm

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Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine using flow cytometry or fluorescence microscopy to assess mixed populations of apoptotic and nonapoptotic cells. Our annexin V conjugates are available as stand-alone reagents, each suitable for 100 flow cytometry assays.
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