End-Point PCR

PCR Enzymes & Mixes

__Standard DNA Polymerase__|__Hot Start DNA Polymerase__|__High Fidelity DNA Polymerase__|
__Other DNA Polymerase__|__Nucleotides__


 

 


 

——  PCR Enzymes Selection by Features  ——

 

Brand 

 

 Item

Features

Product Formats

Template Length

PCR Product

Hot-Start

Proof-
reading

Pre Mix

Direct Loading

UDG Plus

Clean PCR

Bioline

MyTaqTM HS

≤5 kb

A-tailed

Antibody

v

v

MyTaqTM

≤5 kb

v

v

MyFiTM

≤10 kb

Antibody

v

Ranger

≤25 kb

Antibody

v

Velocity

≤10 kb

Blunt end

AccuzymeTM

≤5 kb

v

Enzynomics

nTaq

≤3 kb

A-tailed

v

v

v

nTaq-HOT

≤3 kb

Chemical

v

v

v

v

nTaq-Tenuto

≤10 kb

v

v

v

nTaq-multi HOT

≤3 kb

Chemical

v

v

v

RbTaq™ HOT

≤3 kb

Antibody

v

v

RbTaq™ Tenuto HOT

≤10 kb

Antibody

v

v

nPfu-Special

≤10 kb

Blunt end

nPfu-Forte

≤10 kb

v

v


● Recommended   ○ Sutable

 

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——  PCR Enzymes Selection by Applications  ——

Brand

Item

Routine PCR

Long
PCR

Fast PCR

Multiplex PCR

GC-Rich 

Geno-
typing

High perf. 

Colony PCR

High Fidelity 

Bioline

MyTaqTM HS

MyTaqTM

MyFiTM

Ranger

Velocity

AccuzymeTM

Enzynomics

nTaq

nTaq-HOT

nTaq-Tenuto

nTaq-multi HOT

RbTaq™ HOT

RbTaq™ Tenuto HOT

nPfu-Special

nPfu-Forte


● Recommended   ○ Sutable


 

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Standard DNA Polymerase

 


MyTaq™

 

新世代的DNA polymerase,改善了產量、速度及靈敏度。非常適合用於常規的PCR實驗。
產品包裝形式:

►MyTaq™ DNA Polymerases
►MyTaq™ Red DNA Polymerase  (MyTaq™ with loading dye )
►MyTaq™ Mix  (ready-to-use 2x mix)
►MyTaq™ Red Mix  (ready-to-use 2x mix with loading dye)

 

 


nTaq

 

適合例行性PCR的標準DNA Polymerase
產品包裝形式:

►nTaq
►2X TOPsimple™ PreMIX–nTaq  (ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX–nTaq  (ready-to-use 2x mix with loading dye)
►TOPsimple™ DryMIX–nTaq  (分裝於8連排中的凍乾粉)
►2X TOPsimple™ PreMIX (aliquot)–nTaq  (分裝於8連排中的ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX (aliquot)–nTaq  (分裝於8連排中的ready-to-use 2x mix with loading dye)

 

 
 

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Hot Start DNA Polymerase

 


MyTaq™ HS

 

具Antibody-based熱啟動功能,並改善了產量、速度及效能的高專一性DNA Polymerase。
產品包裝形式:
►MyTaq™ HS DNA Polymerase
►MyTaq™ HS Red DNA Polymerase  (MyTaq™ HS with loading dye)
►MyTaq™ HS Mix  (ready-to-use 2x mix)
►MyTaq™ HS Red Mix  (ready-to-use 2x mix with loading dye)

 

 


RbTaq™ HOT

 

具Antibody-based熱啟動功能的高專一性DNA Polymerase,適合用於易產生非專一性樣品的PCR
產品包裝形式:
►RbTaq™ HOT
►2X RbTaq™ PreMIX-HOT  (ready-to-use 2x mix)
►2X RbTaq™ DyeMIX-HOT  (ready-to-use 2x mix with loading dye)

 

 


MyFi™

 

可合成長達10 kb的DNA,並且非常適合用於GC-Rich sample
產品包裝形式:
►MyFi™ DNA Polymerase
►MyFi™ Mix  (ready-to-use 2x mix)

 

 


RbTaq™ Tenuto HOT

 

兼具Antibody-based熱啟動功能及3'->5’ proofreading功能的DNA Polymerase,錯誤率僅有3.0 X 10-6,適合需要高專一性及精確度的PCR
產品包裝形式:
►RbTaq™ Tenuto HOT
►2X RbTaq™ PreMIX-Tenuto HOT  (ready-to-use 2x mix)
►2X RbTaq™ DyeMIX-Tenuto HOT  (ready-to-use 2x mix with loading dye)

 

 


nTaq-HOT

 

具Chemical-based熱啟動功能的高專一性DNA Polymerase,適合用於易產生非專一性樣品的PCR
產品包裝形式:

►nTaq-HOT
►2X TOPsimple™ PreMIX-HOT (ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX–HOT  (ready-to-use 2x mix with loading dye)
►TOPsimple™ DryMIX-HOT  (分裝於8連排中的凍乾粉)
►2X TOPsimple™ PreMIX (aliquot)–HOT  (分裝於8連排中的ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX (aliquot)–HOT  (分裝於8連排中的ready-to-use 2x mix with loading dye)

 

 

 

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High Fidelity DNA Polymerase

 


VELOCITY

 

3’ – 5’ proofreading exonuclease活性, 錯誤率僅有 4.4 x 10-7
產品包裝形式:
►VELOCITY DNA Polymerase

 

 


nPfu-Forte

 

具3'->5’ proofreading功能的DNA Polymerase,錯誤率僅有2.8 X 10-7,適合高精確度PCR
產品包裝形式:
nPfu-Forte
►2X TOPsimple™ PreMIX–Forte (ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX–Forte (ready-to-use 2x mix with loading dye)
►2X TOPsimple™ DryMIX–Forte (分裝於8連排中的凍乾粉)
►2X TOPsimple™ PreMIX (aliquot)–Forte (分裝於8連排中的ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX (aliquot)–Forte (分裝於8連排中的ready-to-use 2x mix with loading dye) 

 

 


ACCUZYME

 

高效率、具proofreading功能的DNA Polymerase,錯誤率僅有3.0 x 10-6
產品包裝形式:
►ACCUZYME™ DNA Polymerase
►ACCUZYME™ Mix (ready-to-use 2x mix)

 

 


nPfu-Special

 

具3'->5’ proofreading功能的高效率DNA Polymerase,可快速地以15-30 sec/kb進行合成,且錯誤率僅有4.4 X 10-7,非常適合高精確度PCR
產品包裝形式:
►Pfu-Special

 

 

 

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Other DNA Polymerase

 
RANGER
  具3’ – 5’ proofreading功能,適合用於合成10 kb ~ 25 kb的DNA
產品包裝形式:

►RANGER DNA Polymerase
►RANGER Mix  (ready-to-use 2x mix)

 
 
 
nTaq-Tenuto
  具3'->5’ proofreading功能的DNA Polymerase,錯誤率僅有3.0 X 10-6,適合高精確度及長片段PCR
產品包裝形式:
nTaq-Tenuto
►2X TOPsimple™ PreMIX–Tenuto   (ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX–Tenuto  (ready-to-use 2x mix with loading dye)
►TOPsimple™ DryMIX–Tenuto (分裝於8連排中的凍乾粉)
►2X TOPsimple™ PreMIX (aliquot)–Tenuto (分裝於8連排中的ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX (aliquot)–Tenuto (分裝於8連排中的ready-to-use 2x mix with loading dye)
nTaq-Tenuto (Dye plus) (nTaq-Tenuto with loading dye)
nTaq-Tenuto (Uracil+) (nTaq-Tenuto with Uracil)
 
 
nTaq-multi HOT
  適合Multiplex PCR的DNA Polymerase,經測試可同時使用17組primer進行PCR
產品包裝形式:
nTaq-multi HOT
►2X TOPsimple™ PreMIX-multi HOT   (ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX-multi HOT (ready-to-use 2x mix with loading dye)
►2X TOPsimple™ PreMIX (aliquot)–multi HOT (分裝於8連排中的ready-to-use 2x mix)
►2X TOPsimple™ DyeMIX (aliquot)-multi HOT (分裝於8連排中的ready-to-use 2x mix with loading dye)
 

 
 


 
Nucleotides 

 __  dNTP Mix>> __ dNTP Set>> __ dNTP>> 
 
—— dNTP Mix  ———————————————————————————————————————
 
dNTP Mix
  Ready-to-use molecular grade dNTP Mixes containing dATP, dCTP, dGTP and dTTP, designed to save hands-on time for researchers and minimize the possibility of contamination.
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dNTP Mix
  An aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. 
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dNTP Mixture
  This product is a mixture of dATP, dGTP, dCTP, and dTTP (2 mM or 10 mM each) in ultra-pure water (pH 7.0). 
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dNTP Mixture with dUTP
  This product is a mixture of dATP, dGTP, dCTP, dTTP and dUTP (2 mM each) in ultra-pure water (pH 7.0). 
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dNTP Mix
  25 mM each premixed aqueous solution of dATP, dTTP, dCTP, and dGTP.
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——  dNTP Set  ———————————————————————————————————————
 
dNTP Set
  The ready-to-use molecular grade dNTP Sets consist of four separate 100 mM solutions of dATP, dCTP, dGTP and dTTP respectively.
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dNTP set
  This product is a set of each dNTPs (2 mM, 10mM or 100mM).
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dNTP Set
  100 mM aqueous solutions of dATP, dTTP, dCTP and dGTP in separate tubes.
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dUTP Mix
Ready-to-use molecular grade dUTP Mix containing dATP, dCTP, dGTP and dUTP, designed to save hands-on time for researchers and minimize the possibility of contamination.
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——  dNTP   ————————————————————————————————————————
 
dGTP
  The ready-to-use molecular grade 100 mM solution of dGTP as lithium salts in purified water.
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dTTP
  The ready-to-use molecular grade 100 mM solution of dTTP as lithium salts in purified water.
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dCTP
The ready-to-use molecular grade 100 mM solution of dCTP as lithium salts in purified water.
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