3'UTR Target Clones |
Vector | Reporter gene | Tracking gene | 特點 | 搭配分析套組 |
pEZX-MT05 | Gaussia luciferase | Secreted alkaline phosphatase (SEAP) |
不需要裂解細胞,利 用上清液進行活細胞分析 |
Secrete-Pair™ Dual Luminescence Assay Kit |
pEZX-MT06 | Firefly luciferase | Renilla luciferase | 分析前需將細胞裂解 | Luc-Pair™ Luciferase Assay Kit |
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LIN28為已知的miR-125a目標基因, 將GLuc-LIN28-3'-UTR clone轉染到 HEK293細胞,轉染(transfection)後 24小時,偵測GLuc和SEAP的活性。 將只轉染LIN28 3'-UTR target sequence expression clone的GLuc和SEAP的活性比設定為1, 作為標準化的基準。 |
Cat. No. | Product | Description | Size |
LF031 | Secrete-Pair Dual Luminescence Assay Kit (100 rxns) |
Detects Gaussia luciferase (GLuc) |
100 rxns |
LF032 | Secrete-Pair Dual Luminescence Assay Kit (300 rxns) |
300 rxns | |
LF033 | Secrete-Pair Gaussia Luciferase Assay Kit (100 rxns) |
Detects Gaussia luciferase (GLuc) | 100 rxns |
Cat. No. | Product | Description | Size |
LF004 | Luc-Pair™ Duo-Luciferase HS Assay Kit (100rxns) |
For high sensitivity detection of Firefly |
100 rxns |
LF005 | Luc-Pair™ Duo-Luciferase HS Assay Kit (300rxns) |
300 rxns | |
LF001 | Luc-Pair Duo-Luciferase Assay Kits 2.0 (100rxns) |
For enhanced stability detection of Firefly and Renilla luciferase activities. |
100 rxns |
LF002 | Luc-Pair Duo-Luciferase Assay Kits 2.0 (300rxns) |
300 rxns | |
LF013 | Luc-Pair™ Duo-Luciferase HT Assay Kit (10 ml) |
For high-throughput detection of Firefly and Renilla luciferase activities. |
10ml |
LF014 | Luc-Pair™ Duo-Luciferase HT Assay Kit (30 ml) |
30ml |
Precursor expression clones |
Inhibitor expression clones |
Vector-Based Inhibitors |
Synthetic 2'-OMe Inhibitors |
|
---|---|---|
Inhibition | +++++ | ++ |
Specificity | +++++ | +++ |
Stability | +++++ | + |
Durability | Long term | Transient |
Cell Toxicity | - | - |
Delivery to resting and hard-to-transfect cells | +++++ | - |
Vector | Reporter gene |
Selection marker |
Promoter | Type |
---|---|---|---|---|
pEZX-AM01 | mCherry | Puromycin | H1 | Mammalian |
pEZX-AM02 | mCherry | Puromycin | U6 | Mammalian |
pEZX-AM03 | mCherry | Hygromycin | H1 | HIV |
pEZX-AM04 | mCherry | Hygromycin | U6 | HIV |
pEZX-AM04p | mCherry | Puromycin | U6 | HIV |
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mir-125a將來自Gluc-LIN28-3'-UTR clone的螢光素酶活性抑制超過70%(左2)。 將miArrest™ inhibitor clone轉導入細胞後可以阻斷miR-125a對目標基因的抑制作用。 使用劑量越高阻斷效果越好。在最高劑量下(100 ng),GLuc活性已高於對照組,此現象 可歸因於vector-based inhibitor可能已經阻斷內生性miR-125a的調控作用,導致 GLuc-LIN28-3'-UTR transcript的轉譯活性增加。 |
miRNA RT-qPCR kit & Primers |
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3' polyadenylation期間,M-MLV RTase和 一個獨特的Adaptor primer反向轉錄poly A miRNAs。 試劑盒中的qRT-PCR Mix含有SYBR® Green染劑, 用於檢測反轉錄形成的miRNAs。 |
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經過驗證的All-in-One™ miRNA qPCR primer 對靶標miRNA進行特異性擴增並產生特異的溶 解曲線。曲線有良好的一致性。 |
Cat. No. | Product | Description | Size |
QP015 | All-in-One™ miRNA qRT-PCR Detection Kit* |
Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers) |
20 RT and 200 qPCR rxns |
QP016 | All-in-One™ miRNA qRT-PCR Detection Kit* | Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers) | 60 RT and 600 qPCR rxns |
多種 | All-in-One miRNA qPCR Primer | 驗證過人類、小鼠和大鼠miRNA primers | 200 rxns |
miRNA qPCR Array |