DNA/RNA Research


RNase Inhibitor

RiboSafe RNase Inhibitor
代理-Meridian Life Science-免疫學診斷試劑開發用抗體抗原
核糖核酸酶(RNases)無處不在,可通過多種方被引入實驗中而造成實驗污染,例如,抽取RNA的過程中,因手和 pipette tips引起的 RNases污染。RiboSafe RNase Inhibitor 是一種重組蛋白,可以完全抑制各種真核生物RNase,包括RNase A、B和C。
  • 完全抑制RNase A、B和 C
  • 顯著提高RT-PCR靈敏度
  • 不含DNase、RNase和Nickase
  • 不抑制 polymerase/transcriptase 活性
  • 在廣泛的pH值、溫度和DTT濃度範圍內穩定
  • RNA purification
  • cDNA synthesis
  • RNA sequencing
  • in vitro RNA transcription
RiboSafe RNase Inhibitor提供出色的RNA保護
iboSafe inhibits可有效抑制高濃度RNase A
Fig. 1 Inhibition of RNase A by RiboSafe RNase Inhibitor and RNase Inhibitor from another supplier was assessed with the total Yeast RNA assay for the measurement of RNase activity (purple columns) and the pre-incubation-assay (grey columns). RiboSafe RNase Inhibitor blocks RNase A with higher efficiency than other commercially available RNase inhibitors. Fig.2 2 μg of total human HeLa cell RNA was incubated with 20 Units of RiboSafe RNase Inhibitor and 2 ng, 750 pg, 250 pg and 125 pg of RNase A (lanes 1-4) at 37°C for 30 min. Controls were no RiboSafe RNase Inhibitor (lane 5) and total HeLa cell RNA (lane 6) and 2 μg of total human HeLa cell RNA incubated with 125 pg RNase A. HyperLadder 1 kb (M).
RiboSafe RNase抑製劑在高達55°C的溫度下仍具有活性
Fig. 4 2 μg aliquots of mouse total RNA were incubated at 37 °C with RiboSafe RNase Inhibitor and no RNase A (lane 1), no RiboSafe RNase Inhibitor and RNase A (lane 2), or with RiboSafe RNase Inhibitor and RNase A at 37 °C, 42 °C, 45 °C, 50 °C, and 55 °C for 30 minutes (lanes 3—7, respectively, HyperLadder 1kb (M)). Regardless of the temperature, there is no visible RNA degradation when incubated with RiboSafe RNase Inhibitor.
Fig. 3 A two-fold serial dilution of Total HeLa cell RNA (1 μg - 0.075 μg) was reverse transcribed in the presence and in the absence of RiboSafe RNase Inhibitor, followed by the amplification of a 1 kb fragment of the Angiotensin receptor II gene using (lanes 1-10). HyperLadder 1 kb (M).
Cat. No. Product name Pack Size Conc.
BIO-65027 RiboSafe RNase Inhibitor 2500 Units 40 u/μL
BIO-65028 RiboSafe RNase Inhibitor 10,000 Units 40 u/μL