Endotoxin Removal kit
【ProteinArk】內毒素去除管柱試劑組
生物醫療產品中所殘留的內毒素是非常難去除的。許多市售的產品無法有效的降低內毒素含量；同時基於非親和性層析法 (例如：離子交換)，使用Triton X-114來進行相位分離，是一種既耗時又昂貴的方式。Proteus NoEndo™系列產品，使用重力流層析管柱的分離策略並結合離心管柱的速度與易用性，能夠有效的從抗體、重組蛋白、病毒載體的產品中去除高度殘留的內毒素。
| 產品特點 |  |
NoEndo™ µ and M Columns： 採用SelfSeal™ membrane技術，可確保樣本在4度或室溫條件下與樹脂靜置培養的過程中不會通過膜，必須經由離心過程使樣本流入收集管。樣品無須稀釋，可在30分鐘去除內毒素且具有高蛋白回收率（通常大於90％）。 |
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NoEndo™ S and HC Columns： 預先裝填的樹脂濾心並結合FlowGo™技術，可實現樣本與緩衝液在離心的過程中穩定且受控制的流過親和性樹脂，藉由流速的調控來選擇性的捕獲內毒素，可以在2-3小時去除99.9%的內毒素，不影響蛋白質回收率。 |
建議使用的樣本 – 根據您的起始樣本，選用最適用的產品
| NoEndo™ M | NoEndo™ S | NoEndo™ HC | |
| E.coli Expressions | ✓ | ||
| Mammalian Expressions | ✓ | ✓ | |
| Insect Expressions | ✓ | ✓ | |
| Yeast Expressions | ✓ | ✓ | |
| Antibody Samples | ✓ | ✓ | |
| Final Polishing Steps | ✓ | ✓ | ✓ |
出色的產品性能 – 經長時間的處理或進行多次的管柱分離，能大幅度的降低內毒素含量，同時保有高蛋白質回收率
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產品規格
| Spin Columns | NoEndo µ | NoEndo M | NoEndo S | NoEndo HC |
| Typical in situ binding capacity per column | 300-500 EU | 3,000 EU | 30,000 EU | 500,000-1,000,000 EU |
| Typical endotoxin binding capacity | 500-800 EU/ml | 300 EU/ml | 1,500 EU/ml | 30,000 EU/ml |
| Minimum endotoxin levels tested post-column | <0.03 EU/ml | <0.03 EU/ml | <0.05 EU/ml | <0.05 EU/ml |
| Typical endotoxin clearance after 1 pass | - | 3 log reduction | 3 log reduction | |
| Typical endotoxin clearance after 2 passes | - | 4 log reduction | 4 log reduction | |
| Typical endotoxin clearance after 1 hour incubation | 3 log reduction | 2 log reduction | - | - |
| Typical endotoxin clearance after 3 hour incubation | 4 log reduction | 3 log reduction | - | - |
| Maximum sample load volume | 0.6ml | 20 ml | 20 ml | 20 ml |
| Bed volume | 0.01-0.1ml | 0.25 ml | 1 ml | 1.7 ml |
| Resin | NoEndo™ resin | |||
| Bead size range | 100 μm | |||
| Proteus matrix | Cross-linked 6 % agarose | |||
| Recommended working pH | 4-8 | |||
| Color coded end-caps | Supplied in vials | Supplied in vials | Light green | Dark green |
產品選擇
| Kits | Quantity | Product Code |
| Proteus NoEndo™ µ 2 column kit | 0.2 ml resin vial | GEN-NoE2Micro |
| Proteus NoEndo™ µ 24 column kit | 2.4 ml resin vial | GEN-NoE24Micro |
| Proteus NoEndo™ µ 100 column kit | 10 ml resin vial | GEN-NoE100Micro |
| Proteus NoEndoM 2-Column Kit | 0.25 ml resin vial | GEN-NoE2M |
| Proteus NoEndoM 12-Column Kit | 0.25 ml resin vial | GEN-NoE12M |
| Proteus NoEndoM 48-Column Kit | 0.25 ml resin vial | GEN-NoE48M |
| Proteus NoEndoS 2-Column Kit | Resin Cartridges Spin columns | GEN-NoE2S |
| Proteus NoEndoS 12-Column Kit | GEN-NoE12S | |
| Proteus NoEndoS 48-Column Kit | GEN-NoE48S | |
| Proteus NoEndoHC 2-Column Kit | GEN-NoE2HC | |
| Proteus NoEndoHC 12-Column Kit | GEN-NoE12HC | |
| Proteus NoEndoHC 48-Column Kit | GEN-NoE48HC |
| Loose Resin | Quantity | Product Code |
| NoEndo™ Resin (25 ml loose resin) | 25 ml resin | GEN-NoE025ML |
| NoEndo™ Resin (100 ml loose resin) | 100 ml resin | GEN-NoE100ML |
| Accessories | Quantity | Product Code |
| Proteus 1-step batch Midi spin column pack | 8 pack | GEN-1SB08 |
| Proteus 1 ml FliQ column | 1 column | GEN-FliQ1 |
| Proteus 5 ml FliQ column | 1 column | GEN-FliQ5 |
| Proteus 10 ml FliQ column | 1 column | GEN-FliQ10 |
| Proteus 20 ml FliQ column | 1 column | GEN-FliQ20 |
| 10 ml Single step column with bottom frit | 10 pack | 9452086-10 |
| 25 ml Single step column with bottom frit | 10 pack | 9452088-10 |
| 50 ml Single step column with bottom frit | 10 pack | 9452090-10 |
| 100 ml Single step column with bottom frit | 10 pack | 9452092-10 |