體內半胱氨酸 (Cysteine) 水平異常與心血管疾病、亨廷頓舞蹈病、HIV感染和癌症有關。半胱氨酸是許多體外實驗中常見的還原劑。ProFoldin的MicroMolar Cysteine Assay Kit專門設計用於測量半胱氨酸的micromolar濃度,可用於偵測生化反應、藥品和環境水樣品中的半胱氨酸濃度。
測定原理
使用螢光偵測原理,在半胱氨酸的存在下,染劑R53在535 nm波長下的螢光訊號會增強。
*此Assay kit和HEPES buffer相容,若sample中含有 thiol compound (例如DTT)則不適合使用此kit。
References
- Wang LJ et al, Increased serum levels of cysteine in patients with schizophrenia: A potential marker of cognitive function preservation. Schizophrenia Research (2017).
- Ho M.L et al, Silver nanoprism-based paper as ratiometric sensor for extending biothiol detection in serum. New Journal of Chemistry. Oct 30 (2017).
- Jouandin P. et al, Lysosomal cystine efflux opposes mTORC1 reactivation through the TCA cycle. BioRxiv April (2019).
- Gu Q. et al, Effects of acetyl L‐carnitine on zebrafish embryos: Phenotypic and gene expression studies. Journal of Applied Toxicology, 20 July (2020).
- Gu Q. et al, N-acetylcysteine prevents verapamil-induced cardiotoxicity with no effect on the noradrenergic arch-associated neurons in zebrafish. Food and Chemical Toxicology, 5 July (2020).
訂購資訊
貨號 |
產品名稱 |
規格 |
Ex/Em |
CYS200 |
MicroMolar Cysteine Assay Kit |
200 samples |
485 nm/535 nm |
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