Cell Preparation

Filter and Strainer

從血液或組織中分離出單細胞懸浮液,需要移除大量不必要的物質,例如:死細胞、紅血球、細胞團塊、組織碎片等。Miltenyi Biotec提供Strainers、Removal Reagents和FcR Blocking Reagents,可輕鬆且有效率的提高您的樣品純度,避免非專一性結合,使您的實驗不受到干擾。

Filters and Strainers

1. SmartStrainers: 適用在sample dissociation之後過濾單細胞懸浮液,移除組織碎片或細胞團塊
  • 三種篩網尺寸 : 30 µm(黃), 70 µm(紫), 和100 µm(橘)
  • 適用於15 mL 和 50 mL離心管




提高細胞培養結果一致性!​

2. Pre-separation filters: 用於cell separation或Flow cytometry的樣本前處理,移除小的細胞聚合體以及細胞碎片,提升抗體結合的專一性

  • 在螢光/磁性標記細胞、細胞分離、flow、cell sorting前使用,有效率的去除大顆粒細胞團塊&雜質,不會造成液體的阻塞
  • 提供三種篩網尺寸,配合不同類型的細胞樣品:20 µm (綠), 30 µm (黃), 和70 µm (紫)
  • 適用於5 ml和13-15 ml各種規格的管子,及MS、LS、LD、CS、Large Cell和Whole Blood 分選柱
  • 可結合MACS cell separation columns、15 mL or 50 mL tubes與flow tubes
Products Size Order No.
MACS SmartStrainers (30 μm) 100 filters
sterile packed as 4x25 pieces
130-110-915
MACS SmartStrainers (30 μm) 50 filters
sterile, single-packed
130-098-458
MACS SmartStrainers (70 μm) 100 filters
sterile packed as 4x25 pieces
130-110-916
MACS SmartStrainers (70 μm) 50 filters
sterile, single-packed
130-098-462
MACS SmartStrainers (100 μm) 100 filters
sterile packed as 4x25 pieces
130-110-917
MACS SmartStrainers (100 μm) 50 filters
sterile, single-packed
130-098-463
Pre-separation Filters (20 μm) 50 filters 130-101-812
Pre-separation Filters (30 μm) 50 filters 130-041-407
Pre-separation Filters (70 μm) 50 filters 130-095-823

Removal reagents
  • 移除不想要的物質,包含死細胞、紅血球、內毒素
  • 使抗體有更好的專一性結合
  • 提升樣本的純度
 
Products Size Order No.
Basic MicroBeads 2 mL 130-048-001
Dead Cell Removal Kit For 1 x 109 total cells 130-090-101
Endotoxin Removal Beads 3 mL 130-093-657
Endotoxin Removal Beads 5 x 3 mL 130-093-659
Myelin Removal Beads II, human, mouse, rat 2 x 4 mL 130-096-433
Myelin Removal Beads II, human, mouse, rat 4 mL 130-096-733
Myelin Removal Beads II, human, mouse, rat – small size 200 μL 130-096-731
Red Blood Cell Lysis Solution (10X) 50 mL 130-094-183

FcR Blocking Reagents
  • 避免抗體的非專一性結合
  • 提高流式細胞儀分析的專一性
  • 可分別使用在人類或老鼠樣本
 
Products Size Order No.
FcR Blocking Reagents, human 2 mL 130-059-901
FcR Blocking Reagents, mouse 1 mL 130-092-575