Proteomics

Western Blot 西方點墨偵測


Blokers 
       The Blocking Solution (Candor)
       SmartBlock (BSA free) (Candor)
       Block/Wash 20x Concentrate, 50 ml(Cygnus)
       Block/Wash 20x Concentrate, 100 ml(Cygnus)
       Block/Wash 20X Concentrate, 1000 ml (Cygnus)
       BSA-Block (Candor) 
       BSA, Fraction V, Protease Free (GoldBio) 
       DuraUse™ Anti-Spoil Skim Milk Powder, 3 lbs(SignaGen)

ReadyTector all-in-one Solution (Blocker + anti-mouse HRP) 
       ReadyTector® Anti-Mouse-HRP + 10X Wash Buffer (Candor)
       ReadyTector® Wash Buffer 10x (Candor)
       ReadyTector® Chemiluminescent Substrate (ECL) (Candor)

Buffers
       Tris Buffered Saline (TBS) pH 8.0 and 7.6 (Medicago)
       TBS-Tween Tablets - Tris Buffered Saline with Tween™ 20 pH 7.6 (Medicago)
       Sample Buffer (Candor)
       Washing Buffer (Candor)
       LowCross-Buffer (Candor)

Membranes
       PVDF, Immobilon-P Membrane, 0.45 µM (Millipore)
       Nitrocellulose Blotting Membrane, 0.45 µM (Sartorius)
       Blotting Papers (Sartorius)

Stripping Buffer
       Stripping Buffer (Candor)
       BlotFresh™ Western Blot Stripping Reagent (Ver. II) (SignaGen)
       BlotFresh™ Plus Western Blot Stripping Reagent (SignaGen)

Stabilizers
       HRP-Protector (Candor)
       LowCross-HRP (Candor)

Stain
Ponceau S (GoldBio)

Substrate
       LumiGOLD™ ECL Western Blotting Detection Kit (Ver. II) (SignaGen) 
       ReadyTector® Chemiluminescent Substrate (ECL) (Candor)
       BCIP/NBT Substrate, 100 ml (for the detection of alkaline phosphatase in membrane applications)(Cygnus)
       BCIP/NBT Substrate, 1000 ml (for the detection of alkaline phosphatase in membrane applications)(Cygnus)
       TMB Substrate, for Western Blot, 100 ml (Cygnus)
       TMB Substrate, for Western Blot, 1000 ml (Cygnus)
       TMB dihydrochloride, Powder (GoldBio)
       TMB, free base, Powder (GoldBio)