Cloning

Reverse Transcription

__Reverse Transcriptase__|__RNase Inhibitor__|__Nucleotides__|__Primers__|__Water__

 
Selection Guide
 
 
——Reverse Transcriptase Selection Guide ——
Brand Enzynomics Bioline
Item M- MLV  TOPscript™  TOPscript™ II  TOPscript™ III  SuperiorScript II  SuperiorScript III  Tetro Reverse Transcriptase
RNase H activity  Normal Normal Lack Lack Reduced Reduced -
Template Length 5 kb 20 kb  <14 kb <14 kb <12.3 kb <12 kb <9 kb
Reaction temperature 37℃ or 42℃ 42℃~60℃ 42℃~55℃  42℃~55℃ 42℃ 42℃~55℃ 45℃
Concentration 200 U/ul or
1000 U/ul
200 U/ul or
1000 U/ul
200 U/ul or
1000 U/ul
200 U/ul or
1000 U/ul
200 U/ul 200 U/ul 200 U/ul
Other features       7-fold higher fidelity  Improved cDNA yields  Improved cDNA yields
and sensitivity  
Ultra-stable
Similar to         Invitrogen SuperScript II Invitrogen SuperScript III  
Application        
Synthesis of first-strand cDNA v v v   v v v
Primer extension v v v   v v v
cDNA library construction   v     v v v
3' and 5' RACE   v     v v v
Real-Time PCR     v   v v  
Sequencing transcriptomes       v      
RNA splicing analysis       v      
Format Options
Enzyme Only v v
(inculde dNTP)
v
(inculde dNTP)
v
(inculde dNTP)
v
(inculde dNTP)
v
(inculde dNTP)
v
Synthesis Kit v v       v v
Mix type   v          
DryMIX   v          
 

 
►BACK TO TOP

 
Reverse Transcriptase
 
Tetro Reverse Transcriptase
  產品包裝形式:
►Tetro™ Reverse Transcriptase
►Tetro™ cDNA Synthesis Kit 
(套組含有 RTase、Buffer、RNase Inhibitor、dNTP Mix、Primers 和RNase free water )
 
 
M- MLV 
  產品包裝形式:
►M-MLV Reverse Transcriptase
►M-MLV cDNA Synthesis Kit 
(套組含有 RTase、Buffer、RNase Inhibitor、dNTP Mix、Primers 和RNase free water )
 
 
TOPscript™ 
  產品包裝形式:
►TOPscript™ Reverse Transcriptase 

(Supply with dNTP Mix) 
►TOPscript™ cDNA Synthesis Kit 
(套組含有 RTase、Buffer、RNase Inhibitor、dNTP Mix、Primers 和RNase free water )
►TOPscript™ cDNA synthesis Kit (mix type/dT18 plus) 
(mix type, 緩衝液已先預混好dNTP和dT18 primer)
►TOPscript™ cDNA synthesis Kit(mix type/dN6 plus) 
(mix type, 緩衝液已先預混好dNTP和random hexamer primer)
►TOPscript™ RT DryMIX 
(分裝於八連牌中的凍乾粉,已含有RTase、Buffer、RNase Inhibitor、dNTP Mix,不含primer)
►TOPscript™ RT DryMIX(dT18/dN6 plus) 
(分裝於八連牌中的凍乾粉,已含有RTase、 Buffer、RNase Inhibitor、dNTP Mix及 dT18和 random hexamer primers)
►TOPscript™ RT DryMIX (dT18 plus) 
(分裝於八連牌中的凍乾粉,已含有RTase、Buffer、RNase Inhibitor、dNTP Mix及 dT18 primers)
►TOPscript™ RT DryMIX (dN6 plus)
(分裝於八連牌中的凍乾粉,已含有RTase、Buffer、RNase Inhibitor、dNTP Mix及 random hexamer primers)
 
 
TOPscript™ II 
  產品包裝形式:
►TOPscript™ II Reverse Transcriptase (RNase H-) 
(Supply with dNTP Mix)
 
 
TOPscript™ III 
  產品包裝形式:
►TOPscript™ III Reverse Transcriptase (High fidelity) 
(Supply with dNTP Mix)
 
 
SuperiorScript II 
  產品包裝形式:
►SuperiorScript II Reverse Transcriptase 
(Supply with dNTP Mix & DTT)
 
 
SuperiorScript III 
  產品包裝形式:
►SuperiorScript III Reverse Transcriptase 
(Supply with dNTP Mix & DTT)
►SuperiorScript III cDNA Synthesis Kit 
(套組含有 RTase、Buffer、RNase Inhibitor、dNTP Mix、Primers、MgCl2、RNase H、Control RNA、Control RT Primer 和RNase free water )


 
►BACK TO TOP

 
Inhibitors
 
RiboSafe RNase Inhibitor
  RiboSafe RNase Inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C.
Read More>>
 
 
RNase Inhibitor
  RNase inhibitor is from the bacterial strain E. coli and has and activity of >40 U/μL. One unit will reduce the activity of 5 ng of RNase A by 50% in a cytidine 2' 3'Cyclic monophosphate system
Read More>>
 
 
RNase Inhibitor
  This product strongly inhibits major nonspecific ribonucleases that include RNase A, B, C, and human placental RNase.
Read More>>
 
 
RNase Inhibitor, Murine
  RNase Inhibitor, Murine is expressed and purified from E. coli as a recombinant. This product strongly inhibits major nonspecific ribonucleases that include RNase A, B and C.
Read More>>
 
 
 
►BACK TO TOP

 
Nucleotides 

 __ dNTP Mix>> __ dNTP Set>> __ dNTP>> 
 

—— dNTP Mix  ———————————————————————————————————————
 
dNTP Mix
  Ready-to-use molecular grade dNTP Mixes containing dATP, dCTP, dGTP and dTTP, designed to save hands-on time for researchers and minimize the possibility of contamination.
Read More>>
 
 
dNTP Mix
  An aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. 
Read More>>
 
 
dNTP Mixture
  This product is a mixture of dATP, dGTP, dCTP, and dTTP (2 mM or 10 mM each) in ultra-pure water (pH 7.0). 
Read More>>
 
 
dNTP Mixture with dUTP
  This product is a mixture of dATP, dGTP, dCTP, dTTP and dUTP (2 mM each) in ultra-pure water (pH 7.0). 
Read More>>
 
 
dNTP Mix
  25 mM each premixed aqueous solution of dATP, dTTP, dCTP, and dGTP.
Read More>>
 

——  dNTP Set  ———————————————————————————————————————
 
dNTP Set
  The ready-to-use molecular grade dNTP Sets consist of four separate 100 mM solutions of dATP, dCTP, dGTP and dTTP respectively.
Read More>>
 
 
dNTP set
  This product is a set of each dNTPs (2 mM, 10mM or 100mM).
Read More>>
 
 
dNTP Set
  100 mM aqueous solutions of dATP, dTTP, dCTP and dGTP in separate tubes.
Read More>>
 

dUTP Mix
Ready-to-use molecular grade dUTP Mix containing dATP, dCTP, dGTP and dUTP, designed to save hands-on time for researchers and minimize the possibility of contamination.
Read More>>

——  dNTP   ————————————————————————————————————————
 
dGTP
  The ready-to-use molecular grade 100 mM solution of dGTP as lithium salts in purified water.
Read More>>
 
 
dTTP
  The ready-to-use molecular grade 100 mM solution of dTTP as lithium salts in purified water.
Read More>>

dCTP
The ready-to-use molecular grade 100 mM solution of dCTP as lithium salts in purified water.
Read More>>
 

 
►BACK TO TOP

 
Primers

Oligo (dT)18
Read More>>
 
Random Hexamer Primers
Read More>>

Oligo dT (80 μM)
Read More>>
 
Random hexamer (100 μM)
Read More>>


 
►BACK TO TOP

Water
 
Molecular Biology Grade Water Tested to USP Sterile Purified Water Specifications
  Ideal for the preparation of reagents, rinsing glassware and plasticware, and other molecular biology applications where Rnase, Dnase, and Protease-free water is required.
Read More>>