Flow cytometry/Flow antibody |
大小 | |
Width×depth (不含MACS MiniSampler) | 60.5×40 cm |
Width×depth (含MACS MiniSampler) | 60.5×50 cm |
Height (可調整觸控螢幕高度) | 39.3–52 cm |
Laser | Channel | Filter (nm) | Dye or parameter |
紫光雷射405 nm (40mW diode) |
V1 V2 |
450/50 525/50 |
CFP, VioBlue VioFreen |
藍光雷射488 nm (30mW DPSS) |
B1 B2 B3 B4 |
525/50 585/40 655-730 750 LP |
GFP, FITC PE PI, PerCP, PE-Cy5.5 PE-Vio770, PE-Cy7 |
紅光雷射635 nm (20 mW diode) |
R1 R2 |
655-730 750LP |
APC APC-Vio770, APC-Cy7 |
藍光雷射488 nm | FSC SSC |
488/10 488/10 |
Size Granularity |
Laser | Channel | Filter (nm) | Dye or parameter |
紫光雷射405 nm (40mW diode) |
V1 V2 |
450/50 525/50 |
CFP, VioBlue VioGreen |
藍光雷射488 nm (50 mW DPPSS) |
B1 B2 |
525/50 614/50 |
GFP, FITC, YFP PI, Lss-mKate |
黃光雷射561 nm (100 mW diode) |
Y1 Y2 Y3 Y4 |
586/15 615/20 661/20 750LP |
PE, dsRed mCherry, TexasRed PE-Cy5, APC, mKate PE-cy7, APC-cy7, PE-Vio770, APC-Vio770 |
5. 自動細胞標記 (Auto-labeling) MACSQuant Analyzer 10與MACSQuant VYB具有自動細胞標記染色的功能, 是目前在市場上的流式細胞儀之中唯一具備的能力。透過條碼讀取器來輸入所 使用的試劑資料並選取樣本管 (最多可以進行96個樣本標記),軟體有能力自動 進行樣本染色程序,在樣本與樣本染色之間有清洗探針的功能,讓您的樣本不 會交叉汙染。有效的減少手動時間、減少人為誤差和增加數據的再現性與可信 度。 |
6. 自動化程式 MACSQuant是全自動化的流式細胞儀,具有各種自動化的功能,包含: 啟動與暖機程序、校正、樣本標記、清洗探針、螢光補償、流體管路清洗與關機程序 |
最小吸取量 | 25 uL (建議) |
最小樣本大小 | 1 um (建議) |
樣本流速 | 流速25-100 uL/minute,加上自動化維持流速在每秒500、1000、2000個事件 |
最大事件流速 | 最高可到每秒104事件 |
最高的測量速度 | 30分鐘分析一個96孔盤中的樣本 (96個樣本,每個樣本體積10 uL) |
絕對細胞計數 | 精準度+/-5%;再現性 (CV)<5% |
樣本吸取 | 樣本吸取口呈現y/z軸的運動方式,並有自動清洗功能 |
樣本殘留量 | 標準值0.01% |
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MACSQuant Analyzer的絕對細胞計數是出色的 且再現性高。每毫升所含的Counting Beads數量, 經由廠商提供的數據表(A),由MACSQuant Analyzer 計算五管Counting Beads的平均數值(B)。結果顯 MACSQuant Analyzer 計算出的結果與廠商數據 沒有任何差異。 |
將細胞做序列稀釋(1, 0.5, 0.25, 0.1, 0.05倍),經由 MACSQuant測定可得到每毫升的絕對細胞數, 並且數值是成線性關係的。 |
Pre-enrichment的優點 | WorkFlow |
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MACSQuant流式細胞分析儀10![]() |
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MACSQuant流式細胞分析儀VYB![]() |
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Accessories 配件![]() |
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Products: | Capacity/Content/Components | Order No. |
MACSQuant Analyzer 10 | 1 unit | 130–096–343 |
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Products: | Capacity/Content/Components | Order No. |
MACSQuant VYB | 1 unit | 130–096–116 |
Products: | Capacity/Content/Components | Order No. | Description |
Propidium Iodide Solution | 2 ml | 130-093-233 | 用於染色死亡或凋亡細胞 |
7-AAD Staining Solution | 100 tests | 130-111-568 | 用於染色死亡或凋亡細胞 |
FcR Blocking Reagent, human | 2 ml | 130-059-901 | 增加MACS抗體或MACS MicroBeads免疫螢光染色的特異性 |
FcR Blocking Reagent, mouse | 1 ml | 130-092-575 | 增加MACS抗體或MACS MicroBeads免疫螢光染色的特異性 |
Inside Stain Kit | 50 tests | 130-090-477 | 細胞內染色用的fixation和permeabilization試劑,可使用在磁性標記的細胞做細胞內染色 |
One-Step Antibody Biotinylation Kit | 8 rxns | 130-093-385 | 快速免純化的一步法,在單株抗體標記上生物素!讓抗體後續可用在磁性分離或螢光細胞分析上! |
MACSPlex Tc/NK Cell Capture Beads, human,由12種Beads組成,這些Beads都已和專一性的細胞激素捕獲抗體偶聯。可以經由MACSQuant®流式細胞分析儀的B1和B2 Channel檢測到12個Beads群,以不同螢光強度(FITC & PE)來區分。 |
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Jurkat cells cultured with staurosporine (50 nM) for 15 h, were stained with Annexin V-FITC and PI and analyzed by flow cytometry. |
Products | Size | Order No. | Ex/Em (nm) | 偵測法 |
Annexin V-FITC Kit (含有PI #130-093-233 和 Annevin V Binding Buffer #130-092-820) | 100 tests | 130-092-052 | PI: 535/617; FITC: 495/520 |
flow cytometry |
Annexin V-PE | 30 tests | 130-118-499 | 565/578 | flow cytometry |
Annexin V-PE | 100 tests | 130-118-363 | 565/578 | flow cytometry |
Annexin V Binding Buffer (20X Stock Solution) | 25 mL | 130-092-820 | flow cytometry |
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細胞以 (A) PI 或 (B) Viobility Fixable Dye 染色。淺橘色細胞代表凋亡和死細胞 |
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將活PBMC和以95°C,10分鐘熱處理過的人類PBMC和活PBMC混合物用 (A) PI (B) PI和Viobility 488/520 Fixable Dye染色,再以MACSQuant® Flow Cytometry進行分析。 |
Products: | Size | Order No. | Ex/Em (nm) | 偵測法 |
Viobility 405/452 Fixable Dye | 100 tests | 130-109-816● | 405/452 | flow cytometry |
Viobility 405/452 Fixable Dye | 500 tests | 130-110-205● | 405/452 | flow cytometry |
Viobility 405/520 Fixable Dye | 100 tests | 130-109-814● | 405/520 | flow cytometry |
Viobility 405/520 Fixable Dye | 500 tests | 130-110-206● | 405/520 | flow cytometry |
Viobility 488/520 Fixable Dye | 100 tests | 130-109-812● | 488/520 | flow cytometry |
Viobility 488/520 Fixable Dye | 500 tests | 130-110-207● | 488/520 | flow cytometry |
Products: | Capacity/Content/Components | Order No. |
MACS MiniSampler Plus |
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130-105-745 |
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Products: | Capacity/Content/Components | Order No. |
Chill 5 Rack | 1 rack | 130-092-951 |
Chill 5 Racks | 3 racks | 130-097-041 |
Chill 15 Rack | 1 rack | 130-092-952 |
Chill 15 Racks | 3 racks | 130-097-036 |
Chill 50 Rack | 1 rack | 130-092-953 |
Chill 50 Racks | 3 racks | 130-097-037 |
Chill 5, 15, 50 Rack Set |
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130-097-038 |
Chill 96 Rack | 1 rack | 130-094-459 |
Products: | Capacity/Content/Components | Order No. |
MACSQuant Buffer Supply Station – 20 L Bottle |
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130-101-841 |
Products: | Capacity/Content/Components | Order No. |
MACSQuant 16× Running Buffer | 1×1.25 L | 130-111-562 |
MACSQuant 16× Running Buffer | 6×1.25 L | 130-111-747 |
MACSQuant Starting Buffer Kit | 1.5 L MACSQuant Storage Solution 1.5 L MACSQuant Washing Solution 4×1.5 L MACSQuant Running Buffer |
130-094-190 |
MACSQuant Running Buffer | 6×1.5 L | 130-092-747 |
MACSQuant Washing Solution | 6×1.5 L | 130-092-749 |
MACSQuant Storage Solution | 6×1.5 L | 130-092-748 |
MACSQuant Washing & Storage Solution Kit | 3×1.5 L MACSQuant Washing Solution 3×1.5 L MACSQuant Storage Solution |
130-092-801 |
MACS Bleach Solution | 1 L | 130-093-663 |
Products: | Capacity/Content/Components | Order No. |
MACSQuant Columns | 2 columns | 130-094-458 |
Products: | Capacity/Content/Components | Order No. |
MACSQuant Calibration Beads | 5 mL | 130-093-607 |
Bioprocess impurities |
Mycoplasma detection |